PXD061787

PXD061787 is an original dataset announced via ProteomeXchange.

Title	Changes in the Proteome and Phosphoproteome of Zea mays Tissues in Drought Stress Show Plant Tissue Re-sponses from Dehydrins, Carboxylic Acid Metabolism, RNA Splicing and Transcription Factors
Description	Background: Maize is a vital crop, supporting 19.5% of global calorie intake. However, maize is vulnerable to even brief periods of drought which substantially re-duces seed set and therefore yield. Methods: To identify proteins involved in responses of maize to drought, soluble proteins were extracted from leaf and silk tissues of Zea mays and protein abundance and phosphorylation status were quantified relative to well-watered controls. Label-free quantification and phosphopeptide enrichment were applied to the same biological samples and over 300 proteins were identified with sig-nificantly different changes. Results: Proteins known to be involved in drought responses were identified, such as the abscisic acid pathway and transcription factors. Of particular interest is a group of dehydrins quantified at both total protein and phosphopeptide levels, permitting insight into stoichiometry. The biological function of dehydrins in the model plant Arabidopsis thaliana is known to be regulated by phosphorylation. Conclu-sions: Translation of protein function from model plant to crops remains highly chal-lenging because genome duplication has created complex sets of orthologous and ho-mologous proteins. By focusing on proteomic changes during crop stress responses, this work enables the identification of known and novel proteins, substantially aiding the transfer of knowledge from model plants to crops.
HostingRepository	PRIDE
AnnounceDate	2026-05-15
AnnouncementXML	Submission_2026-05-15_02:55:45.291.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georgina Charlton
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: NEWT:4577;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-13 02:57:31	ID requested
1	2026-05-15 02:08:51	announced
⏵ 2	2026-05-15 02:55:45	announced	2026-05-15: Updated project metadata.

10.3390/PROTEOMES14020025;

submitter keyword: None

Alex Jones
contact affiliation	School of Life Sciences, University of Warwick
contact email	alex.jones@warwick.ac.uk
lab head
Georgina Charlton
contact affiliation	University College London
contact email	georgina.charlton@ucl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061787
     1. Label: PRIDE project
     2. Name: Changes in the Proteome and Phosphoproteome of Zea mays Tissues in Drought Stress Show Plant Tissue Re-sponses from Dehydrins, Carboxylic Acid Metabolism, RNA Splicing and Transcription Factors