PXD061778
PXD061778 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protein mass spectrometry analysis of the effect of overexpression of PD-L1 on β-cell derived exosomes components |
| Description | Biomaterial-based implants encapsulating islets or β-cells are desirable regimens for type 1 diabetes (T1D). However, current implants are restricted by poor durable β-cell survival due to immune cell infiltration, graft fibrosis and hypoxia. Programmed cell death-ligand 1 (PD-L1) induces T cell exhaustion, consequently protecting β-cells from autoimmune attack. Herein, we report an implant encapsulating PD-L1-overexpressing-β-cell microspheres (PD-L1 β-MCSs) and Chlorella within alginate hydrogel to control hyperglycemia in T1D. Virtually, PD-L1-β-cell derived exosomes efficaciously induce T cell exhaustion and convert macrophages into M2 phenotype in vitro. And PD-L1 β-MCSs secrete insulin in response to changes in glucose concentration. Furthermore, PD-L1-β-cell microspheres artificial Pancreas (PD-L1-β-MCSs aPancreas) prominently relieve hyperglycemia with less CD4+ T cells, CD8+ T cells, and M1 macrophages infiltration, inflammation and fibrosis deposition. Intriguingly, Chlorella produces oxygen to relieve hypoxia and enables the PD-L1-β-MCSs aPancreas-transplanted mice to achieve sustained normoglycemia, which potentially benefit from both oxygen supplementation and exosomal PD-L1. |
| HostingRepository | iProX |
| AnnounceDate | 2025-03-12 |
| AnnouncementXML | Submission_2025-03-12_19:49:45.975.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xudong Zhang |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-12 19:49:28 | ID requested | |
| ⏵ 1 | 2025-03-12 19:49:46 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: β-cell, PD-L1, exosome, Type 1 diabetes |
Contact List
| Xudong Zhang | |
|---|---|
| contact affiliation | Sun Yat-Sen University |
| contact email | zhangxd56@mail.sysu.edu.cn |
| lab head | |
| Xudong Zhang | |
| contact affiliation | Sun Yat-Sen University |
| contact email | zhangxd56@mail.sysu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
to receive all new ProteomeXchange dataset release announcements!
