PXD061770

PXD061770 is an original dataset announced via ProteomeXchange.

Title	When Less is MORE: Counterintuitive Stoichiometries and Cellular Abundances are Essential for 2 ABC transporters’ function
Description	Prokaryotes acquire essential nutrients primarily through ATP-binding cassette (ABC) importers, 18 consisting of an ATPase, a permease, and a substrate-binding protein. These importers are highly 19 underrepresented in proteomic databases, limiting our knowledge about their cellular copy numbers,20 component stoichiometry, and the mechanistic implications of these parameters. We developed a tailored 21 proteomic approach to compile the most comprehensive dataset to date of the E. coli 'ABC importome'. 22 Functional assays and analysis of deletion strains revealed mechanistic features linking molecular 23 mechanisms to cellular abundances, co-localization, and component stoichiometries. We observed 4-5 24 orders of magnitude variation in import system abundances, with copy numbers tuned to nutrient hierarchies 25 essential for growth. Abundances of substrate-binding proteins are unrelated to their substrate binding 26 affinities but are tightly, yet inversely, correlated with their interaction affinity with permeases. 27 Counterintuitive component stoichiometries are crucial for function, offering insights into the design 28 principles of multi-component protein systems, potentially extending beyond ABC importers.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_17:20:24.905.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamar Ziv
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-12 14:48:30	ID requested
⏵ 1	2025-05-25 17:20:25	announced

Abdullah HQ, Levanon NL, Perach M, Grupper M, Ziv T, Lewinson O, When less is more: Counterintuitive stoichiometries and cellular abundances are essential for ABC transporters' function. Sci Adv, 11(21):eadq7470(2025) [pubmed]

10.1126/sciadv.adq7470;

submitter keyword: Functional-Proteomics,ABC Importers

Oded Lewinson
contact affiliation	Department of Molecular Microbiology, Bruce and Ruth Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
contact email	lewinson@technion.ac.il
lab head
Tamar Ziv
contact affiliation	Technion
contact email	tamarz@technion.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061770

PRIDE project URI

• PRIDE
  1. PXD061770
     1. Label: PRIDE project
     2. Name: When Less is MORE: Counterintuitive Stoichiometries and Cellular Abundances are Essential for 2 ABC transporters’ function