PXD061760

PXD061760 is an original dataset announced via ProteomeXchange.

Title	Importin-7 Promotes Tension-Induced Osteogenesis by Regulating RUNX2 Nuclear Translocation during Orthodontic Tooth Movement
Description	Orthodontic tooth movement (OTM) is primarily driven by alveolar bone remodeling, wherein tension-induced osteogenesis plays a central role. Importin7 (IPO7) has been identified as a highly mechanoresponsive nuclear transport receptor (NTR). However, its role in regulating tension-induced osteogenesis during OTM and its underlying mechanism remains elusive. In the present study, cyclic tensile strain and a rat OTM model were used to investigate the role of IPO7. The results revealed that IPO7 was significantly expressed both in vitro and in vivo following exposure to mechanical stretch. Moreover, IPO7 knockdown inhibited tension-induced osteogenesis in BMSCs. Upon mechanical force stimulation, IPO7 translocated from the cytoplasm to the nucleus. Furthermore, immunoprecipitation (IP) coupled with mass spectrometry (MS) was performed and demonstrated that RUNX2 was one of the IPO7-interacting proteins related to osteogenesis. Although RUNX2 has been established to translocate to the nucleus to facilitate osteogenesis, whether it is IPO7 that regulate RUNX2 nuclear input during tension-induced osteogenesis remains to be determined. Then, the interaction between IPO7 and RUNX2 was further validated via co-immunoprecipitation (co-IP) and colocalization assays in BMSCs using immunofluorescence. Taken together, this study demonstrates that IPO7 promotes tension-induced osteogenesis by regulating the nucleoplasmic localization of RUNX2. Thus, targeting IPO7 may represent a prospective therapeutic strategy for enhancing alveolar bone remodeling and the efficacy of orthodontic treatment.
HostingRepository	PRIDE
AnnounceDate	2025-10-06
AnnouncementXML	Submission_2025-10-05_16:13:19.865.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leilei Zheng
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: NEWT:10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-12 06:29:28	ID requested
⏵ 1	2025-10-05 16:13:20	announced

10.1038/s41598-025-18603-9;

Yang L, Yang G, Yang Q, Zheng L, Importin-7 promotes tension-induced osteogenesis by regulating RUNX2 nuclear translocation during orthodontic tooth movement. Sci Rep, 15(1):33026(2025) [pubmed]

submitter keyword: RUNX2, mechanotransduction,IPO7, tooth movement, nuclear translocation, alveolar bone remodeling

Leilei Zheng
contact affiliation	College of Stomatology, Chongqing Medical University,Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences,Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education,China
contact email	zhengleileicqmu@hospital.cqmu.edu.cn
lab head
Leilei Zheng
contact affiliation	College of Stomatology, Chongqing Medical University
contact email	zhengleileicqmu@hospital.cqmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061760
     1. Label: PRIDE project
     2. Name: Importin-7 Promotes Tension-Induced Osteogenesis by Regulating RUNX2 Nuclear Translocation during Orthodontic Tooth Movement