PXD061719

PXD061719 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Analysis of mini-SHIP1 with pY Peptide by HDX-MS
Description	Hydrogen deuterium exchange mass spectrometry (HDX-MS) was used to analyze how the mini-SHIP1 construct interacts with receptor-derived phosphotryrosine peptides (pY) to probe how these peptides regulate SHIP1 autoinhibition. This HDX-MS helped to identify intramolecular contacts involved in the regulation of SHIP1 autoinibition. Results from this HDX analyzing the monomeric mini-SHIP1 construct are used in tandem with HDX-MS results analyzing the a dimeric SHIP1 construct to confirm the same mechanism of autoinhibtion exists. These results help confirm autoinhibition of monomeric SHIP1 is indeed likely regulated by the same mechanism to that of dimeric SHIP1.
HostingRepository	PRIDE
AnnounceDate	2025-10-15
AnnouncementXML	Submission_2025-10-15_14:08:04.443.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Burke
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	impact HD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-11 12:39:26	ID requested
⏵ 1	2025-10-15 14:08:05	announced

Publication List

10.1016/j.jbc.2025.110788;
Drew EE, Nyvall HG, Parson MAH, Talus RK, Burke JE, Hansen SD, SH2-mediated steric occlusion of the C2 domain regulates autoinhibition of SHIP1 inositol 5-phosphatase. J Biol Chem, ():110788(2025) [pubmed]

10.1016/j.jbc.2025.110788;

Drew EE, Nyvall HG, Parson MAH, Talus RK, Burke JE, Hansen SD, SH2-mediated steric occlusion of the C2 domain regulates autoinhibition of SHIP1 inositol 5-phosphatase. J Biol Chem, ():110788(2025) [pubmed]

Keyword List

submitter keyword: SHIP1, HDX-MS

submitter keyword: SHIP1, HDX-MS

Contact List

John Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
lab head
John Burke
contact affiliation	University of Victoria
contact email	jeburke@uvic.ca
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD061719
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/10/PXD061719

PRIDE project URI

Repository Record List

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