PXD061715
PXD061715 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Calcium Handling in Breast Cancer |
| Description | Extracellular calcium export by the breast ductal epithelium is crucial during lactation and plays a significant role in breast cancer progression. Intraductal calcium deposition is a hallmark of aggressive premalignant lesions. This study tested the hypothesis that microbiome-derived extracellular vesicles (EVs) influence calcium modulation within premalignant breast cancer lesions. Based on the analysis of plasma, serum, saliva, and tissue collected from breast cancer patients and controls (N=150), Lactobacillus rhamnosus (Lr) was chosen as the model microbiota. In a BT-474 human breast cancer cell line monolayer culture under acute calcium stress, Lr EVs enhanced intracellular calcium intake. In a BT474 3D spheroid model under chronic calcium stress, Lr EVs increased extracellular calcium deposition and the mRNA expression of calcium export channel calcium pump isoform 2 (PMCA2) and stromal interaction molecule 1 (STIM1) in a dose-dependent manner. We propose that Lr EVs influence calcium regulation and mineral deposition, thereby affecting premalignant breast cancer progression. |
| HostingRepository | MassIVE |
| AnnounceDate | 2025-03-11 |
| AnnouncementXML | Submission_2025-03-11_13:50:59.611.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Barbara Birkaya |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Lactobacillus; NCBI TaxID: 1578; |
| ModificationList | Carbamidomethyl |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-11 11:35:37 | ID requested | |
| ⏵ 1 | 2025-03-11 13:51:00 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Breast cancer, calcium handling, microbiome, DatasetType:Proteomics |
Contact List
| Alessandra Luchini | |
|---|---|
| contact affiliation | George Mason University |
| contact email | aluchini@gmu.edu |
| lab head | |
| Barbara Birkaya | |
| contact affiliation | George Mason University |
| contact email | bbirkaya@gmu.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v09/MSV000097290/ |
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