PXD061714

PXD061714 is an original dataset announced via ProteomeXchange.

Title	Mechanical cue regulates cargo sorting and export at the Golgi
Description	The secretory pathway is a sophisticated endomembrane machinery designed to transport and deliver proteins and lipids to intracellular organelles and the extracellular space. While the molecular components of the secretory pathway are well understood, less is known about their regulation, especially by mechanical cues. Here, we report that substrate stiffness stimulates conventional secretion. We have unravelled a molecular pathway that links a mechanical cue through Src and FAK kinases to promote the trafficking of secretory proteins out of the Golgi apparatus and prevent their post-Golgi lysosomal degradation. Phosphoproteomic analysis revealed the Golgi-specific Brefeldin A resistance factor 1 (GBF1) as a downstream key mechano-responsive regulator of post-Golgi sorting. Together, our data reveal a molecular regulatory loop in which the matrix stiffness positively regulates cellular secretion via the Src-FAK-GBF1 axis, which can have relevant medical implication in conditions like cancer and fibrosis and their treatment.
HostingRepository	PRIDE
AnnounceDate	2026-01-12
AnnouncementXML	Submission_2026-01-11_17:32:32.022.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Riccardo Rizzo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-11 11:34:09	ID requested
⏵ 1	2026-01-11 17:32:33	announced

10.1002/advs.202506241;

Serafino G, Forciniti S, Scarpa E, Ranieri A, Santorelli L, De Blasi G, Costantini S, Lee EJ, Galeone A, Calcagn, ì A, Pirozzi M, Del Mercato LL, Venditti R, D'Angelo G, Parashuraman S, Verri T, Gigli G, Sztul ES, Grumati P, Rizzello L, Russo D, Rizzo R, Mechanical Cues Regulate Cargo Sorting and Export at the Golgi. Adv Sci (Weinh), 13(1):e06241(2026) [pubmed]

submitter keyword: GBF1, mechano'responsive,Golgi

Riccardo Rizzo
contact affiliation	CNR
contact email	riccardo-rizzo@cnr.it
lab head
Riccardo Rizzo
contact affiliation	Institute of Nanotechnology CNR-NANOTEC
contact email	riccardo-rizzo@cnr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD061714

PRIDE project URI

• PRIDE
  1. PXD061714
     1. Label: PRIDE project
     2. Name: Mechanical cue regulates cargo sorting and export at the Golgi