PXD061694

PXD061694 is an original dataset announced via ProteomeXchange.

Title	Early NK-cell and T-cell dysfunction marks progression to severe dengue in patients with obesity and healthy weight
Description	Dengue is a mosquito-borne virus infection affecting half of the world’s population for which therapies are lacking. The role of T and NK-cells in protection/immunopathogenesis remains unclear for dengue for dengue. We performed a longitudinal phenotypic, functional and transcriptional analyses of T and NK-cells in 124 dengue patients using flow cytometry and single-cell RNA-sequencing. We show that T/NK-cell signatures early in infection discriminate patients who will progressdevelop to severe dengue (SD) from those who do not. These signatures are exacerbated in In patients with overweight/obesity these signatures are exacerbated compared to healthy weight patients, supporting their increased susceptibility to SD. In SD, CD4+/CD8+ T-cells and NK-cells display increased co-inhibitory receptor expression and decreased cytotoxic capacity potential compared to non-SD. Using transcriptional and proteomics approaches we demonstrate decreased Furthermore, type-I Interferon signalling is downregulatedresponses in SD, suggesting defective innate immunity virus-sensing mechanisms may underlie NK/T-cell dysfunction.
HostingRepository	PRIDE
AnnounceDate	2025-05-21
AnnouncementXML	Submission_2025-05-21_08:05:30.896.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Davidson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex reporter+balance reagent acylated N-terminal; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-11 04:39:11	ID requested
⏵ 1	2025-05-21 08:05:31	announced

Dataset with its publication pending

submitter keyword: dengue, CD4+ T-cells, CD8+ T-cells, co-inhibitory receptors, cytotoxic cells, severe dengue biomarkers,NK-cells, type-I IFN

Laura Rivino
contact affiliation	School of Cellular and Molecular Medicine University of Bristol Biomedical Sciences Building University Walk Bristol BS8 1TD, UK
contact email	laura.rivino@bristol.ac.uk
lab head
Andrew Davidson
contact affiliation	University of Bristol, Bristol UK
contact email	andrew.davidson@bristol.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061694

PRIDE project URI

• PRIDE
  1. PXD061694
     1. Label: PRIDE project
     2. Name: Early NK-cell and T-cell dysfunction marks progression to severe dengue in patients with obesity and healthy weight