PXD061671

PXD061671 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis Reveals Protein Degradation in Waterlogged Ancient Leather
Description	Leather artifacts hold significant historical and cultural value in human civilization. During long-term preservation, ancient relics, especially waterlogged leather artifacts, are susceptible to protein degradation. Therefore, analyses of the structure and protein composition of these ancient relics are crucial for their effective conservation. However, comprehensive research in this field is scarce and urgently needed. In this study, systematic investigations of the structures of fresh vegetable-tanned leather, dried leather artifacts, and waterlogged leather artifacts were performed from multiple perspectives. Compared with fresh vegetable-tanned leather and dry leather artifacts, the deterioration of waterlogged leather artifacts resulted in a darkened color, increased brittleness, and reduced fiber structure. Infrared analyses revealed that the characteristic peaks of the amide II and III bands were not present in waterlogged leather artifacts. The species of the leather artifacts were analyzed using enzyme-linked immunosorbent assay (ELISA). Furthermore, comparative proteomics analysis reveals that protein species identified in waterlogged leather artifacts were significantly lower than in fresh vegetable-tanned leather. Type I collagen, along with many structural proteins, was degraded. The degradation of collagen and other structural proteins, along with fiber destruction, caused severe morphological deterioration of waterlogged leather artifacts. A strategy combining species identification by ELISA and proteomics allows the identification of leather proteins with unprecedented levels of speed, sensitivity, and specificity.
HostingRepository	iProX
AnnounceDate	2025-03-10
AnnouncementXML	Submission_2025-03-10_19:46:26.715.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lijie Tang
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-10 19:41:43	ID requested
⏵ 1	2025-03-10 19:46:27	announced

Dataset with its publication pending

submitter keyword: Leather artifact, Proteome, ELISA, Degradation

Huabing Wang
contact affiliation	Zhejiang University
contact email	wanghb@zju.edu.cn
lab head
Lijie Tang
contact affiliation	Zhejiang University
contact email	22317029@zju.edu.cn
dataset submitter

iProX dataset URI