PXD061668

PXD061668 is an original dataset announced via ProteomeXchange.

Title	Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth
Description	Hypoxia frequently occurs during rapid tumour growth. However, how tumour cells adapt to hypoxic stress by remodeling central cellular pathways remains largely unclear. Here, we demonstrated that hypoxia induces casein kinase 2 (CK2)-mediated glucokinase (GCK) S398 phosphorylation, which exposes its nuclear localization signal (NLS) for importin 1 binding and nuclear translocation. Importantly, nuclear GCK interacts with TAZ and functions as a protein kinase that phosphorylates TAZ T346. Phosphorylated TAZ recruits PIN1 for cis–trans isomerization of TAZ, which inhibits the binding of -TrCP to TAZ and -TrCP-mediated TAZ degradation. Activated TAZ-TEAD induces the expression of downstream target genes to promote tumour growth. These findings reveal an instrumental mechanism by which a glycolytic enzyme regulates the Hippo pathway under hypoxic conditions and highlight the moonlighting function of GCK as a protein kinase in modulating TAZ activity and tumour growth.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_21:38:20.179.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gaoxiang Zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-10 18:21:37	ID requested
⏵ 1	2025-07-13 21:38:20	announced

Dataset with its publication pending

submitter keyword: GCK, tumorigenesis, TAZ, PIN1,Hypoxia, phosphorylation

Gaoxiang Zhao
contact affiliation	Qingdao University
contact email	Qchh198@163.com
lab head
Gaoxiang Zhao
contact affiliation	Qingdao University
contact email	QCHH198@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061668
     1. Label: PRIDE project
     2. Name: Nucleus-translocated glucokinase functions as a protein kinase to phosphorylate TAZ and promote tumour growth