PXD061664

PXD061664 is an original dataset announced via ProteomeXchange.

Title	General Trends in the Calnexin-Dependent Expression and Pharmacological Rescue of Clinical CFTR Variants - DDA
Description	Cystic Fibrosis (CF) is a genetic disease caused by mutations in the cystic fibrosis transmembrane conductance regulator gene (CFTR). Though most people with CF have one or two copies of the ΔF508 mutation, there are hundreds of other distinct CF mutations that vary in their mechanistic effects and response to therapeutics. Endogenous chaperones are known to have divergent effects on the druggability of CF variants. Nevertheless, it remains unclear how this proteostatic modulation is related to the underlying mechanistic effects of distinct classes of CF mutations. Here, we survey the effects of a previously discovered effector (calnexin, CANX) on the expression and pharmacological rescue of 235 CF variants using deep mutational scanning. We find that CANX is generally required for robust plasma membrane expression of the CFTR protein- particularly for CF variants that perturb its second nucleotide binding domain. CANX also appears to be critical for the pharmacological rescue of CF variants with poor basal expression. Though corrector selectivity is generally dictated by the properties of mutations, we find that CANX enhances the sensitivity of CF variants within a domain swapped region of membranes spanning domain 2 to the type III corrector VX-445. Together, our findings suggest CANX modulates the later stages of CFTR assembly and disproportionately affects variants bearing mutations within the C-terminal domains. Interestingly, we find that the proteostatic effects of CANX are generally decoupled from changes in CFTR activity. Together, our findings reveal how the proteostasis machinery may shape the variant-specific effects of corrector molecules.
HostingRepository	PRIDE
AnnounceDate	2025-12-04
AnnouncementXML	Submission_2025-12-04_14:38:40.983.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061664
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Olson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-10 16:34:49	ID requested
⏵ 1	2025-12-04 14:38:41	announced

10.7554/ELIFE.107180;

10.6019/PXD061664;

submitter keyword: Variants, Calnexin,CFTR

Lars Plate
contact affiliation	Departments of Chemistry, Biological Sciences, Microbiology and Immunology, Vanderbilt University
contact email	lars.plate@vanderbilt.edu
lab head
John Olson
contact affiliation	Vanderbilt University
contact email	john.a.olson@vanderbilt.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061664
     1. Label: PRIDE project
     2. Name: General Trends in the Calnexin-Dependent Expression and Pharmacological Rescue of Clinical CFTR Variants - DDA