PXD061661

PXD061661 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mitochondrial cell-type-specific profiling: Differential function of mesophyll and guard cells is reflected by their mitochondrial proteome.
Description	Cell specification and the ensueingensuing division of labor enables the accomplishment of complex tasks in multicellular organisms. In plants, mesophyll and guard cells exhibit striking functional, metabolic and mechanophysical differences: mesophyll cells are highly specialized for efficient light-energy conversion, while guard cells regulate gas exchange. Here, we address whether and to what extend the cellular specialization is sustained by mitochondrial diversity between cells. We developed mRACE (RApid CEll-type specific isolation of mitochondria), an innovative method for rapid cell-type-specific mitochondrial isolation. mRACE involves biotin labeling of mitochondria in target cells, followed by affinity-based organelle enrichment using streptavidin-coated microbeads or immobilization on functionalized glass slides. Using mitochondrial activity modulators, we confirmed that mRACE-isolated mitochondria remain physiologically active, validating their suitability for downstream analyses. Applying mRACE, we isolated mitochondria from mesophyll and guard cells and performed a comprehensive proteome analysis. Notably, guard cells exhibit active branched-chain amino acid (BCAA) catabolism, suggesting they exploit this unconventional carbon source for energy. Additionally, both cell types differ in ribosomal protein composition and RNA-editing proteins, highlighting mitochondrial specialization at multiple regulatory levels.
HostingRepository	jPOST
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-14_08:34:53.878.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jürgen Eirich
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue
Instrument	Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-10 12:04:01	ID requested
⏵ 1	2025-07-14 08:34:54	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: mesophyll, guard cells, mitochondria, tissue specific profiling

submitter keyword: mesophyll, guard cells, mitochondria, tissue specific profiling

Contact List

Iris Finkemeier
lab head
Jürgen Eirich
contact affiliation	University of Münster - WWU - Institute for Biology and Biotechnology of Plants
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003686/