PXD061651

PXD061651 is an original dataset announced via ProteomeXchange.

Title	Divergent Plasmodium kinases drive MTOC, kinetochore and axoneme organisation in male gametogenesis
Description	Sexual development and male gamete formation of the malaria parasite in the mosquito midgut is initiated by rapid endomitosis in the activated male gametocyte. This process is highly regulated by protein phosphorylation, specifically by three divergent male-specific protein kinases (PKs): CDPK4, SRPK1 and MAP2. Here, we localise each PK during male gamete formation using live-cell imaging, identify their putative interacting partners by immunoprecipitation, and determine the morphological consequences of their absence using ultrastructure expansion and transmission electron microscopy. Each PK has a distinct location in either the nuclear or cytoplasmic compartment. Protein interaction studies revealed that CDPK4 and MAP2 interact with key drivers of rapid DNA replication, while SRPK1 is involved in RNA translation. The absence of each PK results in severe defects in either microtubule organising centre (MTOC) organisation, kinetochore segregation or axoneme formation. This study reveals the crucial role of these PKs during endomitosis in formation of the flagellated male gamete and uncovers some of their interacting partners that may drive this process.
HostingRepository	PRIDE
AnnounceDate	2025-03-18
AnnouncementXML	Submission_2025-03-18_03:03:43.830.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061651
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-10 07:19:13	ID requested
⏵ 1	2025-03-18 03:03:44	announced

10.6019/PXD061651;

submitter keyword: Plasmodium, Warwick_RTP, Axoneme, MTOC, Gametogenesis, Kinetochore, Kinase

Professor Rita Tewari
contact affiliation	University of Nottingham School of Life Sciences Queens Medical Centre Nottingham NG7 2UH United Kingdom
contact email	rita.tewari@nottingham.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061651
     1. Label: PRIDE project
     2. Name: Divergent Plasmodium kinases drive MTOC, kinetochore and axoneme organisation in male gametogenesis