PXD061641

PXD061641 is an original dataset announced via ProteomeXchange.

Title	Loss of stromal Gαq fosters oral cancer progression
Description	Head and neck squamous cell carcinoma (HNSCC) is a highly aggressive cancer, with limited therapeutic options and a high mortality rate, primarily due to metastasis and recurrence. Tumor-stroma interactions, and namely cancer-associated fibroblasts (CAFs), are pivotal in shaping HNSCC progression. CAFs remodel the extracellular matrix (ECM) and secrete factors and vesicles that promote tumor growth and metastasis. The interplay between autophagy and endosomal/exosomal pathways has been suggested to regulate cellular secretory functions, but their potential involvement in HNSCC progression remains poorly understood. Since we have recently uncovered Gαq as a key modulator of autophagy, we have investigated the impact of Gαq loss on fibroblast functionality and on its crosstalk with oral HNSCC cells. We report that the absence of Gαq rewires murine embryonic fibroblasts towards CAF-like traits, leading to an increased pro-tumorigenic capacity of co-cultured human oral cancer cells through enhanced collagen I deposition and ECM remodeling. Strikingly, fibroblasts lacking Gαq display a shift the balance of intracellular trafficking, degradative and secretory pathways. Exosomes released from Gαq-deficient fibroblasts show a marked enrichment in tumor-growth factor receptors and can facilitate aberrant tumor growth of HNSCC cells. Gαq-silenced fibroblasts promote the formation of "railroad-tracks" structures around HNSCC cells, enhancing their migratory and invasive capabilities both in vitro and in vivo, and reduced Gαq expression in human HNSCC CAFs correlates with enhanced tumor progression. Overall, our data put forward Gαq as a key regulator of the HNSCC tumor microenvironment by modulating fibroblast plasticity and functionality.
HostingRepository	PRIDE
AnnounceDate	2026-03-27
AnnouncementXML	Submission_2026-03-27_04:59:34.377.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan A Lopez
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-10 03:32:45	ID requested
⏵ 1	2026-03-27 04:59:34	announced

Dataset with its publication pending

submitter keyword: Human, exosome, oral cancer,stromal Gαq

Juan A Lopez
contact affiliation	Lab Head Proteomics Unit - CNIC - Madrid, Spain
contact email	jalopez@cnic.es
lab head
Juan A Lopez
contact affiliation	CNIC
contact email	jalopez@cnic.es
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061641
     1. Label: PRIDE project
     2. Name: Loss of stromal Gαq fosters oral cancer progression