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PXD061638

PXD061638 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAstral-DIA Proteomics Deciphers the Molecular Landscape of Human Spermatozoa: A Comprehensive Quantitative Proteomic Characterization of Sperm Function and Fertility Potential
DescriptionMale infertility is fundamentally rooted in developmental defects of germ cells and associated molecular dysregulation, yet the underlying mechanisms remain poorly understood. Data-independent acquisition (DIA) has emerged as a powerful tool in discovery proteomics, enabling the identification of disease biomarkers, therapeutic targets, and molecular pathways with high precision and reproducibility. To analyze the aberrant regulatory events in human sperm proteins associated with male reproductive disorders in a high-throughput, reproducible, and reliable manner, we employed the Orbitrap Astral mass spectrometer, which independently operates Orbitrap Full Scan and Astral MS/MS to generate high-resolution full-scan spectra and high-quality secondary maps. Leveraging the principles of data-independent acquisition (DIA) technology, we constructed the most comprehensive human sperm proteomic expression profile reported to date, encompassing 10,464 proteins, 200,951 precursors, 156,670 modified peptides, and 147,879 peptides.
HostingRepositoryPRIDE
AnnounceDate2025-03-10
AnnouncementXMLSubmission_2025-03-10_03:29:19.383.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRan Kong
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Astral
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-03-10 02:06:33ID requested
12025-03-10 03:29:20announced
Publication List
Perez-Riverol Y, Bandla C, Kundu DJ, Kamatchinathan S, Bai J, Hewapathirana S, John NS, Prakash A, Walzer M, Wang S, Vizca, í, no JA, The PRIDE database at 20 years: 2025 update. Nucleic Acids Res, 53(D1):D543-D553(2025) [pubmed]
10.1093/nar/gkae1011;
Keyword List
submitter keyword: Astral-DIA
Proteomics
Human Spermatozoa
Sperm Function
Contact List
Ran Kong
contact affiliationBiomedical Engineering Department, Peking University, Beijing, China.
contact email597381620@qq.com
lab head
Ran Kong
contact affiliationState Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China
contact email597381620@qq.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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