PXD061638

PXD061638 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Astral-DIA Proteomics Deciphers the Molecular Landscape of Human Spermatozoa: A Comprehensive Quantitative Proteomic Characterization of Sperm Function and Fertility Potential
Description	Male infertility is fundamentally rooted in developmental defects of germ cells and associated molecular dysregulation, yet the underlying mechanisms remain poorly understood. Data-independent acquisition (DIA) has emerged as a powerful tool in discovery proteomics, enabling the identification of disease biomarkers, therapeutic targets, and molecular pathways with high precision and reproducibility. To analyze the aberrant regulatory events in human sperm proteins associated with male reproductive disorders in a high-throughput, reproducible, and reliable manner, we employed the Orbitrap Astral mass spectrometer, which independently operates Orbitrap Full Scan and Astral MS/MS to generate high-resolution full-scan spectra and high-quality secondary maps. Leveraging the principles of data-independent acquisition (DIA) technology, we constructed the most comprehensive human sperm proteomic expression profile reported to date, encompassing 10,464 proteins, 200,951 precursors, 156,670 modified peptides, and 147,879 peptides.
HostingRepository	PRIDE
AnnounceDate	2025-03-10
AnnouncementXML	Submission_2025-03-10_03:29:19.383.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ran Kong
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-10 02:06:33	ID requested
⏵ 1	2025-03-10 03:29:20	announced

Publication List

Perez-Riverol Y, Bandla C, Kundu DJ, Kamatchinathan S, Bai J, Hewapathirana S, John NS, Prakash A, Walzer M, Wang S, Vizca, í, no JA, The PRIDE database at 20 years: 2025 update. Nucleic Acids Res, 53(D1):D543-D553(2025) [pubmed]
10.1093/nar/gkae1011;

Perez-Riverol Y, Bandla C, Kundu DJ, Kamatchinathan S, Bai J, Hewapathirana S, John NS, Prakash A, Walzer M, Wang S, Vizca, í, no JA, The PRIDE database at 20 years: 2025 update. Nucleic Acids Res, 53(D1):D543-D553(2025) [pubmed]

10.1093/nar/gkae1011;

Keyword List

submitter keyword: Astral-DIA
Proteomics
Human Spermatozoa
Sperm Function

submitter keyword: Astral-DIA

Proteomics

Human Spermatozoa

Sperm Function

Contact List

Ran Kong
contact affiliation	Biomedical Engineering Department, Peking University, Beijing, China.
contact email	597381620@qq.com
lab head
Ran Kong
contact affiliation	State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China
contact email	597381620@qq.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD061638
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD061638

PRIDE project URI

Repository Record List

[ + ]