PXD061632

PXD061632 is an original dataset announced via ProteomeXchange.

Title	A fully automated All-in-Tip platform for integrated sample pretreatment advancing plasma proteomics
Description	Large-scale plasma proteomics studies can advance the human proteome atlas mapping, facilitating precision medicine, but are constrained by the low efficiency of sample pretreatment. Here, we reported a fully automated plasma proteomic platform based on the All-in-Tip, enabling the whole sample pretreatment to be performed at room temperature within 1 hour. The All-in-Tip used functional macroporous nanoparticles (NPs) to form protein corona in 5 minutes to narrow the abundance range of plasma proteins and the spatial confinement of macroporous NPs to offer efficient digestion in 5 minutes. The All-in-Tip workflow is highly coupled with automation to achieve high reproducibility (correlation > 0.95, median coefficient of variation < 30%) proteomic profiling. Our strategy was validated in the early-stage lung adenocarcinoma cohort, annotating over 5100 protein groups, screening 353 differentially expressed proteins, and distinguishing early-stage lung adenocarcinoma from pulmonary benign diseases with an AUC of 0.89. The All-in-Tip workflow offers high-throughput, robust and deep profiling, which meets the requirements of large-scale clinical cohorts and thus promises to advance the elucidation of disease mechanisms and new biomarker discovery.
HostingRepository	iProX
AnnounceDate	2025-03-10
AnnouncementXML	Submission_2025-03-09_22:58:46.524.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yating Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Bos taurus; NCBI TaxID: 9913; scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-09 22:58:29	ID requested
⏵ 1	2025-03-09 22:58:46	announced

Dataset with its publication pending

submitter keyword: proteomics, All-in-Tip, automation, sample pretreatment, plasma

Jingjing Wan
contact affiliation	East China Normal University
contact email	jjwan@chem.ecnu.edu.cn
lab head
Yating Wang
contact affiliation	East China Normal University
contact email	52274300022@stu.ecnu.edu.cn
dataset submitter

iProX dataset URI