PXD061623

PXD061623 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	LGI3 promotes the progression of TFE3-rearranged renal cell carcinoma through GEMIN6/AURKB axis
Description	Transcription factor enhancer 3-rearranged renal cell carcinoma (TFE3-RCC) is characterized by its aggressive nature, limited treatment options, and poor prognosis. However, the downstream targets of TFE3 fusion protein responsible for its tumorigenesis and progression remain unclear. Here, we demonstrated that leucine-rich repeat LGI family member 3 (LGI3) as a direct target of TFE3 fusion protein. TFE3 fusion protein can bind to the promoter of LGI3 and then activate its transcription. Importantly, LGI3 contributes to the proliferation, migration, and invasion of TFE3-RCC. Mechanistically, LGI3 interacts with gem nuclear organelle-associated protein 6 (GEMIN6) and inhibits its degradation via decreasing its ubiquitination. GEMIN6 upregulation promotes the mRNA maturation of Aurora B kinase (AURKB), thereby promoting the progression of TFE3-RCC. Importantly, drugs targeting GEMIN6 or AURKB significantly suppressed the growth of TFE3-RCC cells or organoids. In human TFE3-RCC tissues, LGI3 is highly expressed and positively correlated with GEMIN6 and AURKB. Overall, we revealed a novel mechanism underlying the progression of TFE3-RCC and provided potential new therapeutic targets.
HostingRepository	PRIDE
AnnounceDate	2025-03-30
AnnouncementXML	Submission_2025-03-30_02:40:20.085.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Junxiao Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-09 19:08:42	ID requested
⏵ 1	2025-03-30 02:40:20	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: TFE3-RCC,LGI3, Organoid, Therapeutic targets, Transcriptional regulation

submitter keyword: TFE3-RCC,LGI3, Organoid, Therapeutic targets, Transcriptional regulation

Contact List

Xiubin Li
contact affiliation	The Third Medical Center, Chinese PLA General Hospital
contact email	klootair@163.com
lab head
Junxiao Liu
contact affiliation	The Third Medical Center, Chinese PLA General Hospital
contact email	liujunxiaodoctor@163.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD061623
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD061623

PRIDE project URI

Repository Record List

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