PXD061617

PXD061617 is an original dataset announced via ProteomeXchange.

Title	Circadian proteomics of the mouse Suprachiasmatic Nucleus
Description	Spike-in heavy labeled protein lysate was generated by culturing SCN slices for 6 week in the precense of heavy arginine and lysine. SCN slices where harvested at 5 diffrent time points during one circadian cycle. The SCN slices where lysed and spiked with heavy SCN lysate. The samples were processed with the FASP protocol and and proteins quantified by LC-MS/MS using the MaxQuant software. To identify proteins with a circadian change in abundance with use JTK-cycle.
HostingRepository	PRIDE
AnnounceDate	2025-06-09
AnnouncementXML	Submission_2025-06-08_16:06:47.740.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Toke Hansen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; 6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-09 11:54:42	ID requested
⏵ 1	2025-06-08 16:06:48	announced

10.1002/glia.70018;

Patton AP, Krogager TP, Maywood ES, Smyllie NJ, Morris EL, Skehel M, Hastings MH, Multi-Omic Analysis Reveals Astrocytic Annexin-A2 as Critical for Network-Level Circadian Timekeeping in the Suprachiasmatic Nucleus. Glia, 73(7):1483-1501(2025) [pubmed]

submitter keyword: SCN, Circadian,Mouse, LC-MS/MS

Michael Harvey Hastings
contact affiliation	Division of Neurobiology, Laboratory of Molecular Biology, Cambridge, UK
contact email	mha@mrc-lmb.cam.ac.uk
lab head
Toke Hansen
contact affiliation	Danish Center for Particle Therapy
contact email	toke.krogager@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD061617

PRIDE project URI

• PRIDE
  1. PXD061617
     1. Label: PRIDE project
     2. Name: Circadian proteomics of the mouse Suprachiasmatic Nucleus