PXD061616

PXD061616 is an original dataset announced via ProteomeXchange.

Title	Dynamic subcellular proteomics identifies novel regulators of adipocyte insulin action
Description	Insulin acts on adipocytes to control whole-body glucose and lipid metabolism by inhibiting lipolysis and increasing glucose uptake. Regulation of these processes by insulin signalling is dependent on changes in protein localisation, for example GLUT4 delivery to the plasma membrane (PM). However, the extent of spatial proteomic changes in response to insulin signalling, and role these play in the cellular insulin response, is incomplete. Here, we use subcellular proteomics to map insulin-stimulated protein relocalisation in adipocytes on a cell-wide scale. Approximately 10% of proteins identified exhibited altered subcellular localisation in response to insulin. Since the PM was a major site of proteome remodelling in response to insulin, we performed additional PM proteome profiling to quantify insulin-responsive changes at the cell surface. These orthologous proteomics approaches revealed insulin-stimulated redistribution of the uncharacterised protein C3ORF18 to the PM. Studies in adipocytes depleted of C3ORF18 suggest this protein is required for maximal insulin signalling. Overall, our data provide an accessible resource of insulin-regulated changes in adipocyte protein localisation, and impetus for further work on how changes in protein localisation contribute to the cellular insulin response.
HostingRepository	PRIDE
AnnounceDate	2026-02-06
AnnouncementXML	Submission_2026-02-06_08:37:14.665.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Weekes
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-09 09:51:57	ID requested
⏵ 1	2026-02-06 08:37:15	announced

10.1101/2025.03.13.642412;

submitter keyword: proteomics,insulin, adipocyte, subcellular, plasma membrane

Michael Weekes, University of Cambridge
contact affiliation	Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge, CB2 0XY, UK
contact email	mpw1001@cam.ac.uk
lab head
Michael Weekes
contact affiliation	University of Cambridge
contact email	mpw1001@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061616
     1. Label: PRIDE project
     2. Name: Dynamic subcellular proteomics identifies novel regulators of adipocyte insulin action