PXD061610

PXD061610 is an original dataset announced via ProteomeXchange.

Title	GAPDH inhibition in A549 and H358 lung cancer cells
Description	Human lung cancer cell lines A549 and H358 were obtained from (Biobank of Medical University of Graz) and cultured in RPMI 1640 (R0883; Sigma) supplemented with 10% fetal bovine serum (FBS; Gibco), 5 mM glutamine and 5 mM Pen Step, and sub-cultured regularly. For siRNA mediated knock-down of GAPDH, 150,000 A549 and H358 cells were seeded in triplicates of a six-well plate and transfected upon reaching ca. 60% confluency with silencerTM siRNA kit targeting GAPDH or non-target control (AM4605; Thermo), using RNAiMax (Thermo) and according to the manufacturer’s instructions for 6-well plate scale. For GAPDH inhibition, 150.000 A549 and H358 cells were seeded in six replicates per condition, then treated with 10 µM koningic acid (KA) or vehicle control (dmso). 48 h post transfection or 24 h post KA treatment, cells were harvested in 500 µl of harvesting solution (80% methanol in 50 mM ammonium acetate with 2.5 mM N-ethylmaleimide) and processed according to one-pot redox approach.
HostingRepository	PRIDE
AnnounceDate	2025-05-12
AnnouncementXML	Submission_2025-05-12_02:54:26.015.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tamara Tomin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-08 13:46:39	ID requested
⏵ 1	2025-05-12 02:54:26	announced

Dataset with its publication pending

submitter keyword: human, koningic acid,GAPDH

Ruth Birner-Gruenberger
contact affiliation	Univ.Prof. PD DI Dr.techn. Professor for Bioanalytics TU Wien Faculty of Technical Chemistry Institute of Chemical Engineering and Analytics Department of Imaging and Analytical Chemistry Research Group Bioanalytics Getreidemarkt 9/E164 1060 Vienna Austria
contact email	ruth.birner-gruenberger@tuwien.ac.at
lab head
Tamara Tomin
contact affiliation	Senior Scientist at Institute of Chemical Technologies and Analytics Faculty of Technical Chemistry, TU Wien Getreidemarkt 9/164, 1060 Vienna
contact email	tamara.tomin@tuwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061610

PRIDE project URI

• PRIDE
  1. PXD061610
     1. Label: PRIDE project
     2. Name: GAPDH inhibition in A549 and H358 lung cancer cells