PXD061602

PXD061602 is an original dataset announced via ProteomeXchange.

Title	Affinity-purification of HA-tagged NPHP4 wild-type and S862N mutant in SK-N-BE2 cells
Description	The alphaherpesvirus Varicella-Zoster Virus (VZV) infects most humans. It causes chickenpox, shingles and rare severe central nervous system (CNS) pathologies. To gain molecular insights in the virus’ pathobiology, we conducted a proteomic survey on the interactions and effects of 64 VZV proteins, and VZV infection-induced perturbations, in neuronal SK-N-BE2 cells. This identified 900 interactors and 3618 regulated host proteins (https://varizonet.innatelab.org). Data integration and functional validations of the identified host proteins unveiled NPHP4 as VZV restriction factor. Combining whole exome of patients with VZV-associated CNS pathologies identified the NPHP4 S862N variant. Importantly, the S862N mutation impaired NPHP4's antiviral activity, and supressed its interactions with 14-3-3 proteins, as measured by affinity-purification.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-14_09:42:14.474.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Virginie Girault
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-08 04:58:07	ID requested
⏵ 1	2025-07-14 09:42:14	announced

10.1038/S41564-025-02068-7;

submitter keyword: LC-MS/MS, NPHP4, APMS

Andreas Pichlmair
contact affiliation	Institute of Virology, Technical University of Munich, Munich, Germany
contact email	andreas.pichlmair@tum.de
lab head
Virginie Girault
contact affiliation	Institute of Virology - Technical University Munich
contact email	v.girault@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD061602

PRIDE project URI

• PRIDE
  1. PXD061602
     1. Label: PRIDE project
     2. Name: Affinity-purification of HA-tagged NPHP4 wild-type and S862N mutant in SK-N-BE2 cells