PXD061597

PXD061597 is an original dataset announced via ProteomeXchange.

Title	Structural basis of antiphage defence by an ATPase-associated reverse transcriptase
Description	Reverse transcriptases (RTs) have well-established roles in the replication and spread of retroviruses and retrotransposons. However, recent evidence suggests that RTs have been conscripted by cells for diverse roles in antiviral defence. Here we determine structures of a type I-A retron, which explain how RNA, DNA, RT, HNH-nuclease and four molecules of an SMC-family ATPase assemble into a 364 kDa complex that provides phage defence. We show that phage-encoded nucleases trigger degradation of the retron-associated DNA, leading to disassembly of the retron and activation of the HNH nuclease. The HNH nuclease cleaves tRNASer, stalling protein synthesis and arresting viral replication. Taken together, these data reveal diverse and paradoxical roles for RTs in the perpetuation and elimination of genetic parasites.
HostingRepository	PRIDE
AnnounceDate	2025-05-23
AnnouncementXML	Submission_2025-05-23_14:06:44.955.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061597
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jerrin Thomas George
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-07 16:44:09	ID requested
⏵ 1	2025-05-23 14:06:45	announced

10.6019/PXD061597;

submitter keyword: ATPase,Bacterial Immunity, Retron

Blake Wiedenheft
contact affiliation	Department of Microbiology and Cell Biology, Montana State Univeristy
contact email	bwiedenheft@gmail.com
lab head
Jerrin Thomas George
contact affiliation	Montana State University
contact email	jerrin.thomasgeorge@montana.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061597
     1. Label: PRIDE project
     2. Name: Structural basis of antiphage defence by an ATPase-associated reverse transcriptase