PXD061589

PXD061589 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of cardiac regeneration in the leopard gecko, Eublepharis macularius
Description	Various species of teleost fish, amphibians, and neonatal mammals can regenerate their hearts following injury. Certain processes, including extracellular matrix remodelling, a switch from oxidative to glycolytic metabolism and sarcomere disorganization are believed to be critical to heart regeneration. However, whether all regenerating species follow the same pathway to regeneration is not known. Here, we use mass-spectrometry based proteomics to characterize the cellular processes that occur in the heart of a reptile, the leopard gecko (Eublepharis macularius) following injury. The hearts were damaged using a cryoprobe and wound sites were sampled at 3-, 14-, 30- and 100-days post injury (dpi). The proteomes of these were compared to those from sham operated geckos. Overall, 1116 proteins were identified in the wound sites and 579 were differentially expressed across at least two time points. This includes an increase in agrin at 14-dpi, a protein thought to be required for cardiomyocyte proliferation and heart regeneration. Interaction network analysis indicates that there is enrichment of Gene Ontology (GO) terms related to transcription and translation by 14-dpi, but a concurrent decrease in GO terms related to oxidative and glycolytic metabolism, and sarcomere organization. Importantly, GO terms related to the structure and function of mitochondria as well as sarcomere organization were enriched at 100-dpi compared to 3-dpi, and 14-dpi. There were no GO terms identified as differing between sham and 100 dpi hearts, suggesting full regeneration. This work indicates that the gecko heart can regenerate and that this involves reorganization of cellular pathways associated with mitosis, energy production and contractile function.
HostingRepository	PRIDE
AnnounceDate	2025-12-12
AnnouncementXML	Submission_2025-12-12_06:40:26.785.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jared Shaftoe
SpeciesList	scientific name: Eublepharis macularius; NCBI TaxID: NEWT:481883;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-07 10:00:31	ID requested
⏵ 1	2025-12-12 06:40:27	announced

10.1016/J.CBPA.2024.111769;

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Leopard gecko, Heart regeneration, Cryoinjury, Mass Spec

Todd E. Gillis
contact affiliation	Professor, Department of Integrative Biology, College of Biological Sciences, University of Guelph
contact email	tgillis@uoguelph.ca
lab head
Jared Shaftoe
contact affiliation	University of Guelph
contact email	wjshaftoe@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061589
     1. Label: PRIDE project
     2. Name: Proteomic analysis of cardiac regeneration in the leopard gecko, Eublepharis macularius