⮝ Full datasets listing
PXD061552
PXD061552 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics changes in K7M2 mouse osteosarcoma cell line after ezrin gene is deleted and then restored with either a wildtype, T567A mutant, or T567D mutant |
| Description | We first knocked out the endogenous ezrin expression in K7M2 mouse osteosarcoma cell line by using CRISPR/Cas9 gene editing at the EZRIN gene locus. We then stably rescued the ezrin expression by stably transfecting them with either a wildtype, a T567A mutant, or a T567D mutant EZRIN cDNA carrying vector or left the cells unrestored by transfecting only an empty vector. The resulting cell lines were referred here as COL3WT, COL3A, COL3D, or COL3E. Each cell line has three replicates for the proteomics analysis. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-19 |
| AnnouncementXML | Submission_2026-03-19_06:34:39.922.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Emre Deniz |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | nanoACQUITY UPLC; Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-06 15:37:54 | ID requested | |
| ⏵ 1 | 2026-03-19 06:34:40 | announced |
Publication List
| Deniz E, Tiwari P, Tiwari PB, Glasgow E, Harris BT, Tiwari A, Hou C, Ma J, Toretsky JA, Ü, ren A, The unphosphorylated, closed form of ezrin binds to RNA to maintain a metastatic phenotype in osteosarcoma cells. Sci Signal, 19(929):eady8367(2026) [pubmed] |
Keyword List
| submitter keyword: proteomics, osteosarcoma, k7m2, mouse, DatasetType:Proteomics, ezrin, t567a, t567d |
Contact List
| Aykut Uren | |
|---|---|
| contact affiliation | Georgetown University Medical Center |
| contact email | au26@georgetown.edu |
| lab head | |
| Emre Deniz | |
| contact affiliation | Georgetown University |
| contact email | ed828@georgetown.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097250/ |
