⮝ Full datasets listing
PXD061550
PXD061550 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | LKB1 regulates JNK-dependent stress signaling and apoptotic dependency of KRAS-mutant lung cancers |
| Description | KRAS-mutant lung cancers frequently have inactivating mutations in LKB1, which enhances tumor growth, metastasis, and immune evasion. This study shows that LKB1 suppresses JNK stress signaling, and its loss in KRAS-mutant cells leads to JNK hyperactivation and apoptotic rewiring, making them sensitive to MCL-1 inhibition. These findings highlight a previously unknown role of LKB1 in stress signaling and suggest a potential therapeutic approach for LKB1-deficient KRAS-mutant lung cancers. Multiplexed proteomics and phosphoproteomics was performed using TMT tags and the SPS3 method on an Orbitrap Fusion Lumos instrument. Sample key is as follows: H2030 EV TRAM (126), H2030 LKB1 VEH (127N), H358 sgGFP TRAM (127C), H358 sgLKB1 VEH (128N), H2030 LKB1K87I (kd) TRAM (128C), H358 sgLKB1 TRAM (129N), H358 sgGFP VEH (129C), H2030 LKB1 TRAM (130N), H2030 LKB1K87I (kd) VEH (130C), H2030 EV VEH (131N) |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-03 |
| AnnouncementXML | Submission_2026-03-03_13:10:50.739.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Johannes Kreuzer |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | unknown modification; unknown modification; unknown modification; unknown modification |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-06 15:35:59 | ID requested | |
| ⏵ 1 | 2026-03-03 13:10:51 | announced |
Publication List
| Li C, Syed MU, Nimbalkar A, Shen Y, Vieira MD, Fraser C, Inde Z, Qin X, Ouyang J, Kreuzer J, Clark SE, Kelley G, Hensley EM, Morris R, Lazaro R, Belmonte B, Oh A, Walcott M, Nabel CS, Caenepeel S, Saiki AY, Rex K, Lipford JR, Heist RS, Lin JJ, Haas W, Sarosiek K, Hughes PE, Hata AN, LKB1 regulates JNK-dependent stress signaling and apoptotic dependency of KRAS-mutant lung cancers. Nat Commun, 16(1):4112(2025) [pubmed] |
Keyword List
| submitter keyword: KRAS, STK11/LKB1, MCL-1, non-small cell lung cancer, apoptosis, Phosphoproteomics, Orbitrap Fusion Lumos, TMT, DatasetType:Proteomics |
Contact List
| Chendi Li | |
|---|---|
| contact affiliation | Massachusetts General Hospital and Harvard Medical School |
| contact email | cli32@mgh.harvard.edu |
| lab head | |
| Aaron N Hata | |
| contact affiliation | Massachusetts General Hospital and Harvard Medical School |
| contact email | ahata@mgh.harvard.edu |
| lab head | |
| Wilhelm Haas | |
| contact affiliation | Massachusetts General Hospital and Harvard Medical School |
| contact email | whaas@mgh.harvard.edu |
| lab head | |
| Johannes Kreuzer | |
| contact affiliation | MGH |
| contact email | JKREUZER@mgh.harvard.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097246/ |
