PXD061546

PXD061546 is an original dataset announced via ProteomeXchange.

Title	A systems view on DNA damage response kinetics
Description	A tightly regulated DNA damage response is critical to the overall integrity of the genome. Here, we combine transcriptomics and proteomics to study DNA damage response kinetics across well-established treatments in the ciliate Tetrahymena thermophila. This extensive data set of 6 conditions (HU, MMS, IR, HP, cisplatin and UV) and 7 time points (from 0 to 8 hours) integrates over 250 transcriptome and proteome measurements. We observed upregulation of known DNA repair proteins as well as a global dynamic response of not yet characterized transcripts and proteins. Using artificial neural networks, we classify different expression trends in response to the damaging agents. These networks revealed both a core and specific global dynamic response to the different genotoxic stressors, highlighting unexpected pathway crosstalk. In addition to the comprehensive analysis presented here, the data can be explored via an accessible user interface at https://butterlab.imb-mainz.de/TtDDR/. Ultimately, our study provides novel insights into the DNA damage response kinetics in Tetrahymena.
HostingRepository	PRIDE
AnnounceDate	2026-01-06
AnnouncementXML	Submission_2026-01-06_03:48:00.922.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Tetrahymena thermophila; NCBI TaxID: NEWT:5911;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-06 12:48:30	ID requested
⏵ 1	2026-01-06 03:48:01	announced

Dataset with its publication pending

submitter keyword: LC-MS/MS

Falk Butter
contact affiliation	Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
lab head
F Butter
contact affiliation	Quantitative Proteomics Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061546
     1. Label: PRIDE project
     2. Name: A systems view on DNA damage response kinetics