PXD061537

PXD061537 is an original dataset announced via ProteomeXchange.

Title	Chemoproteomic Approach for Identifying Nuclear Arsenite-binding Proteins
Description	Trivalent arsenic [As(III)], the predominant environmental form, poses significant health risks, including cancer, diabetes, and cardiovascular and neurodegenerative diseases, through contaminated food and water ingestion. Studies have explored As(III) toxicity mechanisms, particularly its displacement of Zn(II) in zinc-binding proteins, but identifying direct As(III)-binding proteins remains crucial. Given the nucleic acid binding capacity of many zinc-binding proteins, we hypothesized that nuclear As(III)-binding proteins could reveal key toxicity targets. Using a quantitative proteomics approach, we performed affinity pull-down with a biotin-As(III) probe on nuclear protein lysate. We identified splicing factor 1 (SF1) as a direct As(III)-binding protein and demonstrated that this interaction modulates mRNA splicing in human cells.
HostingRepository	PRIDE
AnnounceDate	2025-05-03
AnnouncementXML	Submission_2025-05-02_21:15:00.671.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shiyuan Guo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-06 10:34:00	ID requested
⏵ 1	2025-05-02 21:15:01	announced

10.1021/ACS.CHEMRESTOX.5C00107;

submitter keyword: HEK293T, proteomic, LC-MS/MS

Yinsheng Wang
contact affiliation	Department of Chemistry, University of California, Riverside
contact email	yinsheng@ucr.edu
lab head
Shiyuan Guo
contact affiliation	Genetics, Genomics and Bioinformatics Graduate Program, University of California, Riverside
contact email	sguo039@ucr.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061537

PRIDE project URI

• PRIDE
  1. PXD061537
     1. Label: PRIDE project
     2. Name: Chemoproteomic Approach for Identifying Nuclear Arsenite-binding Proteins