PXD061533

PXD061533 is an original dataset announced via ProteomeXchange.

Title	Polydopamine nanoparticles (PDNPs) to remotely modulate the activity of differentiated SH-SY5Y cells and differentiated C2C12 cells
Description	The precise control of cell activity is crucial for understanding and potentially treating many disorders. Focusing on neurons and myotubes, recent advancements in nanotechnology have introduced photoresponsive nanoparticles as a novel tool for modulating cell function with high spatial and temporal resolution. This approach offers a non-invasive alternative to traditional stimulation approaches, reducing potential tissue damage and improving the specificity of cell activation. Here, we introduce an approach envisioning fully organic polydopamine nanoparticles (PDNPs) to remotely modulate the activity of differentiated SH-SY5Y cells and differentiated C2C12 cells, via near-infrared (NIR) laser stimulation. Confocal microscopy imaging revealed the possibility of thermally activating individual neuron-like cells, eliciting a significant cellular response characterized by the generation of calcium transients and the following release of the neurotransmitter acetylcholine. Similarly, we demonstrated the possibility of precisely triggering muscle contraction of single myotubes. Additionally, we investigated the antioxidant properties of PDNPs, demonstrating their capacity to prevent an increment of oxidative stress levels related to an increase in intracellular temperature. Moreover, proteomic analysis revealed that PDNP treatment enhances neuronal plasticity and nervous system maturation while promoting muscle growth and preserving its functional integrity, underscoring its potential to support both neural and musculoskeletal development. Eventually, the effect of the NIR laser irradiation in the presence of PDNPs in neuron-like cells was successfully evaluated ex vivo on brains of Drosophila melanogaster, genetically modified to express the fluorescent calcium indicator jGCaMP7c.
HostingRepository	PRIDE
AnnounceDate	2025-04-23
AnnouncementXML	Submission_2025-04-23_00:45:42.564.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrea Armirotti
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-06 09:16:07	ID requested
⏵ 1	2025-04-23 00:45:42	announced

10.1021/ACSNANO.5C04181;

submitter keyword: neurons,polydopamine, NIR, nanoparticles

Andrea Armirotti
contact affiliation	Istituto Italiano di Tecnologia Via Morego 30, 16163 Genova Italy
contact email	andrea.armirotti@iit.it
lab head
Andrea Armirotti
contact affiliation	Analytical Chemistry Lab, Fondazione Istituto Italiano di Tecnologia
contact email	andrea.armirotti@iit.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061533
     1. Label: PRIDE project
     2. Name: Polydopamine nanoparticles (PDNPs) to remotely modulate the activity of differentiated SH-SY5Y cells and differentiated C2C12 cells