PXD061512

PXD061512 is an original dataset announced via ProteomeXchange.

Title	Bovine Liver Membrane Preparation
Description	We optimized an SDS depletion protocol involving KCl to precipitate the surfactant from intact proteins in the presence of urea at high pH. Provided is a comparison of 4 samples preciipitated at low pH (8) vs high pH (12), with (8 M) vs without (0 M) urea.
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-03_16:06:24.101.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alan Doucette
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-05 11:35:06	ID requested
⏵ 1	2025-08-03 16:06:24	announced

Iranpour T, Mirimba M, Shenouda C, Lynch A, Doucette AA, SDS Depletion from Intact Membrane Proteins by KCl Precipitation Ahead of Mass Spectrometry Analysis. Proteomes, 13(3):(2025) [pubmed]

10.3390/proteomes13030030;

submitter keyword: urea, pH,SDS depleted, KCl preciptation

Alan A Doucette
contact affiliation	Department of Chemistry, Dalhousie University, Halifax, Nova Scotia, Canada
contact email	alan.doucette@dal.ca
lab head
Alan Doucette
contact affiliation	Dalhousie University
contact email	alan.doucette@dal.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD061512

PRIDE project URI

• PRIDE
  1. PXD061512
     1. Label: PRIDE project
     2. Name: Bovine Liver Membrane Preparation