PXD061503 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Conformational landscapes of rigid and flexible molecules explored with variable temperature ion mobility-mass spectrometry |
| Description | Variable temperature ion mobility-mass spectrometry (VT-IM-MS) can measure the effect of temperature on conformational landscapes. To delineate collision effects from structural change we report measurements using molecules with different degrees of rigidity namely: poly (L-lysine) (PLL) dendrimer, ubiquitin, β-casein and α-synuclein from 190-350 K. The CCS of PLL dendrimer varies with temperature consistent with collision theory, by contrast, the structure of each protein alters with notable restructuring at 350 K and 250 K, following predicted in vitro stability curves. At 210 K and 190 K we kinetically trap unfolding intermediates. For alpha-synuclein, the 13+ ions present two distinct conformers and VT-IM-MS measurements allow us to calculate the transition rate and activation energies of their conversion. These data exemplify the capacity of VT-IM-MS to provide insights to thermodynamics of conformational restructuring. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-23 |
| AnnouncementXML | Submission_2026-02-22_16:11:44.117.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jason M D Kalapothakis |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q-Tof Ultima |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2025-03-05 06:57:22 | ID requested | |
| ⏵ 1 | 2026-02-22 16:11:45 | announced | |
Publication List
| Wang X, Norgate E, Dai J, Benoit F, Bristow T, England RM, Kalapothakis JMD, Barran PE, Conformational landscapes of rigid and flexible molecules explored with variable temperature ion mobility-mass spectrometry. Nat Commun, 16(1):4183(2025) [pubmed] |
| 10.1038/s41467-025-59065-x; |
Keyword List
| submitter keyword: Variable Temperature, beta-casein, alpha-synuclein, poly-L-lysine, IM-MS, Ubiquitin |
Contact List
| Perdita E. Barran |
|---|
| contact affiliation | Manchester Institute of Biotechnology School of Natural Schiences University of Manchester M1 7DN Manchester United Kingdom |
| contact email | perdita.barran@manchester.ac.uk |
| lab head | |
| Jason M D Kalapothakis |
|---|
| contact affiliation | University of Manchester |
| contact email | jason.kalapothakis@manchester.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD061503
- Label: PRIDE project
- Name: Conformational landscapes of rigid and flexible molecules explored with variable temperature ion mobility-mass spectrometry
