PXD061497

PXD061497 is an original dataset announced via ProteomeXchange.

Title	Flagellar proteins of Phaeobacter inhibens DSM 17395 and selected mutants
Description	Flagellar motility is crucial for the swim-and-stick lifestyle of Rhodobacterales and plays an important role for bacterial-algal interactions. This alphaproteobacterial order contains three distinct types of flagellar gene clusters (FGCs) for the formation of a functional flagellum. Swimming of the marine model organism Phaeobacter inhibens DSM 17395 (Roseobacteraceae) is mediated by the archetypal fla1 flagellum. Screening of 13,000 transposon mutants on soft agar plates revealed that 40 genes, including four genes encoding conserved but not yet characterized proteins (CP1-4) within the FGC, are essential for motility. Exoproteome analyses of P. inhibens DSM 17395 wildtype and 10 transposon mutants, i.e., ΔmotB, ΔCP1, ΔCP2, ΔCP3, ΔCP4, ΔfliK, ΔfliI, ΔflgH, ΔctrA, Δ65kb plasmid were conducted.
HostingRepository	PRIDE
AnnounceDate	2025-06-23
AnnouncementXML	Submission_2025-06-23_00:12:16.085.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lars Wöhlbrand
SpeciesList	scientific name: Bacteria; NCBI TaxID: NCBITaxon:2; scientific name: Phaeobacter gallaeciensis (strain ATCC 700781 / DSM 17395 / CIP 105210 / NBRC 16654 / BS107) (Roseobacter gallaeciensis); NCBI TaxID: 391619;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	amaZon Speed ETD

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-05 02:16:22	ID requested
⏵ 1	2025-06-23 00:12:16	announced

10.1128/MSYSTEMS.00419-25;

submitter keyword: LC-MSMS, Flagella,Roseobacter

Ralf Rabus
contact affiliation	General and molecular microbiology, Institute for chemistry and biology of the marine environment (ICBM), Carl von Ossietzky University of Oldenburg, Oldenburg, Germany
contact email	rabus@icbm.de
lab head
Lars Wöhlbrand
contact affiliation	General and Molecular Microbiology
contact email	lars.woehlbrand@uni-oldenburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD061497

PRIDE project URI

• PRIDE
  1. PXD061497
     1. Label: PRIDE project
     2. Name: Flagellar proteins of Phaeobacter inhibens DSM 17395 and selected mutants