⮝ Full datasets listing
PXD061495
PXD061495 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Salmon nasal cartilage proteoglycan up-regulates Listeria monocytogenese-mediated immune response in mice |
| Description | The proteomic analysis of liver samples from mice administered proteoglycan (PG) for 9 days or untreated control mice. Liver samples from mice administered PG for 9 days or untreated control mice (n = 6 per group) were homogenized in PBS. The proteins were precipitated using acetone. The supernatant from the homogenized liver was transferred into two-milliliter microcentrifuge tubes, and four times the sample volume of cold (-20oC) acetone was added. After the vortex, the tubes were incubated overnight at -20oC. Acetone was removed by centrifugation at 14,000 x g for 10 min at 4oC, then the remaining acetone was allowed to evaporate at room temperature for 30 min. The protein pellet was dissolved in PBS, and protein concentration was measured using Bradford protein assay. Proteins were first denatured using 50% trifluoroethanol and reduced with 4 mM dithiothreitol. Alkylation of free cysteine residues was performed before trypsin digestion, and the desalted peptides were subsequently separated through liquid chromatography. A mass spectrometer was used to analyze the samples. The resulting spectra were compared against Mus musculus proteins to assess differences in protein expression between PG-treated mice and non-treated controls (NC), determining the relative abundance of proteins in each group. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-03-05 |
| AnnouncementXML | Submission_2026-03-04_07:00:21.253.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yota Tatara |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | S-carboxamidomethyl-L-cysteine; N6-acetyl-L-lysine; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); 2-pyrrolidone-5-carboxylic acid (Glu); L-methionine sulfoxide; phosphorylated L-histidine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | TripleTOF 6600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2025-03-05 01:55:07 | ID requested | |
| ⏵ 1 | 2026-03-04 07:00:22 | announced |
Publication List
| Nakane A, Subsomwong P, Takahashi T, Ito K, Asano K, -mediated immune response in mice. Curr Res Microb Sci, 9():100465(2025) [pubmed] |
Keyword List
| submitter keyword: proteoglycan, Listeria monocytogenese, Salmon nasal cartilage |
Contact List
| Krisana Asano | |
|---|---|
| lab head | |
| Yota Tatara | |
| contact affiliation | Hirosaki University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003673/ |
