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PXD061468

PXD061468 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIsoform-specific Patterns of Phosphorylation on Axonemal Dynein Heavy Chains
DescriptionAxonemal dyneins power ciliary motility and phosphorylation of key intermediate and light chain components affects the regulation and properties of these motors in very distantly related organisms. It is also known that many axonemal dynein heavy chains are subject to this post-translational modification although this has been little studied. Here we examine axonemal dynein heavy chains from a broad range of ciliated eukaryotes and identify phosphorylated sites embedded within various kinase recognition motifs such as those for PKA, PKC and casein kinase II. Mapping these sites onto discrete heavy chain types reveals class-specific locations apparently mediated by different kinases. For example, we find that all Chlamydomonas  heavy chain phosphorylation sites are in an extended loop derived from AAA5 that arches over the coiled-coil buttress which in turn interacts with the microtubule-binding stalk. In contrast, most sites in the monomeric inner arm dyneins occur very close to the N-terminus and may be involved in assembly processes. In Chlamydomonas, the two cilia (termed cis and trans) exhibit different intrinsic beat frequencies and we identify cilium-specific phosphorylation patterns on both the  heavy chain and outer arm docking complex consistent with differential regulation of these motors in the two organelles.
HostingRepositoryPRIDE
AnnounceDate2025-04-25
AnnouncementXMLSubmission_2025-04-25_11:50:00.504.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJeremy Balsbaugh
SpeciesList scientific name: Chlamydomonas sp. HS-5; NCBI TaxID: 108458;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-03-04 11:36:24ID requested
12025-04-25 11:50:01announced
Publication List
Sakato-Antoku M, Patel-King RS, Inaba K, Balsbaugh JL, King SM, Isoform-specific phosphorylation of axonemal dynein heavy chains. Mol Biol Cell, 36(6):ar67(2025) [pubmed]
10.1091/mbc.e25-03-0116;
Keyword List
submitter keyword: dynein, axoneme, chlamydomonas, phosphorylation
Contact List
Dr. Jeremy Balsbaugh
contact affiliationProteomics & Metabolomics Facility, Center for Open Research Resources & Equipment University of Connecticut Storrs, CT 06269
contact emailjeremy.balsbaugh@uconn.edu
lab head
Jeremy Balsbaugh
contact affiliationUniversity of Connecticut
contact emailjeremy.balsbaugh@uconn.edu
dataset submitter
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