PXD061468

PXD061468 is an original dataset announced via ProteomeXchange.

Title	Isoform-specific Patterns of Phosphorylation on Axonemal Dynein Heavy Chains
Description	Axonemal dyneins power ciliary motility and phosphorylation of key intermediate and light chain components affects the regulation and properties of these motors in very distantly related organisms. It is also known that many axonemal dynein heavy chains are subject to this post-translational modification although this has been little studied. Here we examine axonemal dynein heavy chains from a broad range of ciliated eukaryotes and identify phosphorylated sites embedded within various kinase recognition motifs such as those for PKA, PKC and casein kinase II. Mapping these sites onto discrete heavy chain types reveals class-specific locations apparently mediated by different kinases. For example, we find that all Chlamydomonas  heavy chain phosphorylation sites are in an extended loop derived from AAA5 that arches over the coiled-coil buttress which in turn interacts with the microtubule-binding stalk. In contrast, most sites in the monomeric inner arm dyneins occur very close to the N-terminus and may be involved in assembly processes. In Chlamydomonas, the two cilia (termed cis and trans) exhibit different intrinsic beat frequencies and we identify cilium-specific phosphorylation patterns on both the  heavy chain and outer arm docking complex consistent with differential regulation of these motors in the two organelles.
HostingRepository	PRIDE
AnnounceDate	2025-04-25
AnnouncementXML	Submission_2025-04-25_11:50:00.504.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jeremy Balsbaugh
SpeciesList	scientific name: Chlamydomonas sp. HS-5; NCBI TaxID: 108458;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-04 11:36:24	ID requested
⏵ 1	2025-04-25 11:50:01	announced

Sakato-Antoku M, Patel-King RS, Inaba K, Balsbaugh JL, King SM, Isoform-specific phosphorylation of axonemal dynein heavy chains. Mol Biol Cell, 36(6):ar67(2025) [pubmed]

10.1091/mbc.e25-03-0116;

submitter keyword: dynein, axoneme, chlamydomonas, phosphorylation

Dr. Jeremy Balsbaugh
contact affiliation	Proteomics & Metabolomics Facility, Center for Open Research Resources & Equipment University of Connecticut Storrs, CT 06269
contact email	jeremy.balsbaugh@uconn.edu
lab head
Jeremy Balsbaugh
contact affiliation	University of Connecticut
contact email	jeremy.balsbaugh@uconn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD061468

PRIDE project URI

• PRIDE
  1. PXD061468
     1. Label: PRIDE project
     2. Name: Isoform-specific Patterns of Phosphorylation on Axonemal Dynein Heavy Chains