PXD061451

PXD061451 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	NEDD4L BioID - Hyperosmosis_Rotin-Raught
Description	Dataset contains LC-MS results from BioID of NEDD4L fused with miniTurbo and expressed in T-REx Flp-In HEK293 cells. Following Tet-dependent induction and addition of biotin, cells were collected, lysed in RIPA, cleared and incubated with streptavidin sepharose. Captured proteins were digested with trypsin and analyzed on an Easy nLC 1200 pump in-line with a Q Exactive HF instrument operated in ESI positive mode. Spectral count results were analyzed with SAINT by comparison with miniTurbo-only BioID samples to generate a list of proximity interactors.
HostingRepository	MassIVE
AnnounceDate	2025-09-20
AnnouncementXML	Submission_2025-09-20_09:43:05.818.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jonathan St-Germain
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Acetyl; Deamidated
Instrument	Q Exactive HF

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-04 05:34:02	ID requested
⏵ 1	2025-09-20 09:43:06	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: NEDD4L, T-REx Flp-In HEK293, miniTurbo, BioID, LC-MS, biotin, streptavidin, DatasetType:Proteomics

submitter keyword: NEDD4L, T-REx Flp-In HEK293, miniTurbo, BioID, LC-MS, biotin, streptavidin, DatasetType:Proteomics

Contact List

Brian Raught
contact affiliation	Princess Margaret Cancer Centre, University Health Network
contact email	brian.raught@uhn.ca
lab head
Jonathan St-Germain
contact affiliation	Princess Margaret Cancer Centre
contact email	jonstgermain@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097232/

MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v09/MSV000097232/