PXD061436

PXD061436 is an original dataset announced via ProteomeXchange.

Title	Ultradeep N-glycoproteome Atlas of Mouse Reveals Spatiotemporal Signatures of Brain Aging and Neurodegenerative Diseases
Description	The current depth of site-specific N-glycoproteomics is insufficient to fully characterize glycosylation events in biological samples. Herein, we achieved an ultradeep and precision analysis of the N-glycoproteome of mouse tissues by integrating multiple workflows. The largest N-glycoproteomic dataset to date was established on mice, which contained 91,972 precursor glycopeptides, 62,216 glycoforms, 8,939 glycosites and 4,563 glycoproteins. The database consisted of 6.8 million glyco-spectra (containing oxonium ions), among which 160,928 were high-quality spectra with confident N-glycopeptide identifications. The large-scale and high-quality dataset enhanced the performance of current artificial intelligence models for glycopeptide tandem spectrum prediction. Using this ultradeep dataset, we observed tissue specific microheterogeneity and functional implications of protein glycosylation in mice. Furthermore, the region-resolved brain N-glycoproteomes for Alzheimer's Diseases, Parkinson Disease and aging mice revealed the spatiotemporal signatures and distinct pathological functions of the N-glycoproteins. A comprehensive database resource of experimental N-glycoproteomic data from this study and previous literatures were further established. This N-glycoproteome atlas serves as a promising tool for revealing the role of protein glycosylation in biological systems.
HostingRepository	iProX
AnnounceDate	2025-03-04
AnnouncementXML	Submission_2025-03-31_19:36:31.190.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	WeiWei Zhao
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-04 00:33:03	ID requested
⏵ 1	2025-03-31 19:36:31	announced

Dataset with its publication pending

submitter keyword: Glycoproteomics

Mass Spectrometry

Database

Site-specific Glycopeptides

Pan Fang
contact affiliation	Soochow University
contact email	pfang@suda.edu.cn
lab head
WeiWei Zhao
contact affiliation	Soochow University
contact email	weiwei_vivi@126.com
dataset submitter

iProX dataset URI