PXD061424

PXD061424 is an original dataset announced via ProteomeXchange.

Title	Interactors and targeted substrates of UBL7 in mouse testis and sperm
Description	Spermiogenesis is a tightly regulated process to produce mature sperm cells. The ubiquitin-proteasome system (UPS) plays a crucial role in controlling protein half-life and is essential for spermiogenesis. In this study, we demonstrate that UBL7, a testis-enriched UBL-UBA protein, is indispensable for sperm formation. UBL7 interacts with the valosin-containing protein (VCP) complex and proteasomes, and shuttles substrates between them. Notably, UBL7 slows down the degradation rates of substrates involved in endoplasmic reticulum-associated degradation (ERAD) within cells. Through tandem mass tag (TMT)-labeled quantitative proteomics of sperm from Ubl7+/- and Ubl7-/- epididymis, we found lots of proteins were decreased in Ubl7-/- sperm, including critical factors involved in ATP metabolism and glycolytic process. Through a two-step immunoprecipitation method, we identify several essential factors in spermatids that are protected by UBL7, including factors involved in the development of manchette (such as IFT140), head-tail coupling apparatus (such as SPATA20) and cytoplasmic droplets (such as HK1 and SLC2a3). In summary, our findings highlight UBL7 as a guardian that protects crucial factors from excessive degradation and thereby ensures successful spermiogenesis.
HostingRepository	iProX
AnnounceDate	2025-03-04
AnnouncementXML	Submission_2025-05-19_00:23:42.352.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tianyi Yuan
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2025-03-03 19:32:28	ID requested
1	2025-03-31 20:08:54	announced
⏵ 2	2025-05-19 00:23:43	announced	2025-05-19: Add Publication.

Yuan T, Yang J, Xu D, Li H, Min W, Wang F, UBL7 is indispensable for spermiogenesis through protecting critical factors from excessive degradation by proteasomes. Nat Commun, 16(1):3803(2025) [pubmed]

submitter keyword: UBL7, sperm, UBL-UBA protein

Fengchao Wang
contact affiliation	National Institute of Biological Sciences, Beijing
contact email	wangfengchao160415@163.com
lab head
Tianyi Yuan
contact affiliation	National Institute of Biological Sciences, Beijing
contact email	yuantianyi@nibs.ac.cn
dataset submitter

iProX dataset URI