⮝ Full datasets listing

PXD061414

PXD061414 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of post-translationally modified MHC class 1-associated peptides
DescriptionOver the past three decades, the Hunt laboratory has developed advancements in mass spectrometry-based technologies to enable the identification of peptides bound to major histocompatibility complex (MHC) molecules. The MHC class I processing pathway is responsible for presenting these peptides to circulating cytotoxic T cells, allowing them to recognize and eliminate malignant cells, many of which have aberrant signaling. Professor Hunt hypothesized that due to the dysregulation in phosphorylation in cancer, that abnormal phosphopeptides are likely presented by this pathway, and went on to discover the first phosphopeptide presented by the MHC processing pathway. Thereafter, the laboratory continued to sequence MHC-associated phosphopeptides and contributed several improved methods for their enrichment, detection, and sequencing. This manuscript summarizes the most recent advancements in identification of modified MHC-associated peptides and includes the cumulative list of phosphopeptides sequenced by the Hunt lab. Further, many other post-translational modifications (PTMs) were found to modify MHC peptides, including O-GlcNAcylation, methylation, and kynurenine; in total, we present here a list of 2,450 MHC-associated PTM peptides. Many of these were disease specific and found across several patients, thus highlighting their potential as cancer immunotherapy targets. We are sharing this list with the field in hopes that it might be used in investigating this potential. Overall, the Hunt lab’s contributions have significantly advanced our understanding of antigen presentation and dysregulation of PTMs, supporting modern immunotherapy and vaccine development efforts.
HostingRepositoryPRIDE
AnnounceDate2025-04-15
AnnouncementXMLSubmission_2025-04-15_10:15:14.866.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD061414
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJeffrey Shabanowitz
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmethylated glutamic acid; methylated aspartic acid; phosphorylated residue; alpha-carboxyl methylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-03-03 14:16:50ID requested
12025-04-15 10:15:15announced
Publication List
10.6019/PXD061414;
10.1016/J.MCPRO.2025.100971;
Keyword List
submitter keyword: IMAC, post-translational modification, cancer immunotherapy, immunopeptidomics,MHC associated peptides
Contact List
Jeffrey Shabanowitz
contact affiliationUniversity of Virginia, Department of Chemistry
contact emailjs4c@virginia.edu
lab head
Jeffrey Shabanowitz
contact affiliationUniversity of Virginia
contact emailjs4c@virginia.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD061414
PRIDE project URI
Repository Record List
[ + ]