PXD061319

PXD061319 is an original dataset announced via ProteomeXchange.

Title	Dehydroglutathione to induce glutathione modification in protein
Description	Protein S-glutathionylation is one of the major cysteine oxidations regulating redox signaling and oxidative stress. In this study, we developed a glutathione-derived chemical tool, namely dehydroglutathione, that can introduce a non-reducible glutathionylation mimic to protein, which can be utilized for analyzing the functional effects of glutathionylation. Dehydroglutathione reacts with protein cysteines to form a thio-ether based glutathione modification in proteins. We applied dehydroglutathione to fatty acid binding protein 5 (FABP5). Dehydroglutathione modification in FABP5 was analyzed by LC-MS/MS to identify the cysteine sites of modification.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:06:07.041.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Young-Hoon Ahn
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-28 10:26:38	ID requested
⏵ 1	2025-07-13 16:06:07	announced

10.1039/d5cb00052a;

Oppong D, Padmavathi R, Kukulage DSK, Shivamadhu MC, Newberry EA, Faustino AM, Tang HY, Ahn YH, Dehydroglutathione, a glutathione derivative to introduce non-reversible glutathionylation. RSC Chem Biol, 6(7):1156-1164(2025) [pubmed]

submitter keyword: glutathionylation, FABP5,Dehydroglutathione

Young-Hoon Ahn
contact affiliation	Drexel University, Department of Chemistry
contact email	ya426@drexel.edu
lab head
Young-Hoon Ahn
contact affiliation	Drexel University
contact email	ya426@drexel.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD061319

PRIDE project URI

• PRIDE
  1. PXD061319
     1. Label: PRIDE project
     2. Name: Dehydroglutathione to induce glutathione modification in protein