PXD061305

PXD061305 is an original dataset announced via ProteomeXchange.

Title	Characterization of N-terminal acetylation of α-hemoglobin stabilizing protein (AHSP) by top-down high resolution mass spectrometry of oral fluids from human preterm newborns.
Description	The project reports the identification in oral fluid samples of preterm newborns of AHSP (Alpha-hemoglobin Stabilizing Protein), an erythroid-specific protein forming stable complexes with free α-hemoglobin preventing its harmful aggregation and pro-oxidant activity during red blood cell development, following nanoHPLC-high resolution MS proteomic analysis in top-down approach and the determination of its primary structure. Full scan MS spectra deconvolution revealed a monoisotopic mass of the protein inconsistent with the previously reported sequence. The analysis of the MS fragmentation spectra of the protein multicharged ion at 980.29 m/z [M+12H]12+ evidenced a shift of the experimental b-ions which extended from the amino terminus, suggesting the presence of an N-terminal modification in the protein, i.e., the elimination of the iMet1 and the acetylation of the Nα-terminal Ala2, which was confirmed by comparison of the theoretical and experimental MS fragmentation pattern.
HostingRepository	PRIDE
AnnounceDate	2025-07-18
AnnouncementXML	Submission_2025-07-18_01:41:46.555.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Claudia Desiderio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-28 06:24:20	ID requested
⏵ 1	2025-07-18 01:41:46	announced

10.1002/RCM.10107;

submitter keyword: Oral fluid top-down proteomics

Preterm newborn biomarkers

Alpha-hemoglobin stabilizing protein (AHSP)

LC-MS

neonatal hematological disorders

Barbara Manconi
contact affiliation	Department of Life and Environmental Sciences, Statal University of Cagliari,09042 Monserrato(CA),Italy
contact email	bmanconi@unica.it
lab head
Claudia Desiderio
contact affiliation	Consiglio Nazionale delle Ricerche - Istituto di Scienze e Tecnologie Chimiche (SCITEC) "Giulio Natta"
contact email	claudia.desiderio@cnr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD061305

PRIDE project URI

• PRIDE
  1. PXD061305
     1. Label: PRIDE project
     2. Name: Characterization of N-terminal acetylation of α-hemoglobin stabilizing protein (AHSP) by top-down high resolution mass spectrometry of oral fluids from human preterm newborns.