PXD061303 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | In Vitro Psilocybin Synthesis by Co-Immobilized Enzymes |
Description | Advanced clinical trials investigate the Psilocybe magic mushroom natural product psilocybin as a treatment against major depressive disorder. Currently, synthetic material is used to meet the demand for legitimate pharmaceutical purposes. Here, we report an in vitro approach to biocatalytically produce psilocybin on a solid-phase matrix charged with five covalently bound biosynthetic enzymes. These enzymes include three Psilocybe enzymes: IasA, an engineered L-tryptophan decarboxylase/aromatic aldehyde synthase, the 4-hydroxytryptamine kinase PsiK and the norbaeocystin methyltransferase PsiM, along with Escherichia coli nucleosidase MtnN and adenine deaminase Ade. In a proof-of-principle experiment, this enzyme-charged resin allowed for quantitative turnover of 4-hydroxy-L-tryptophan into psilocybin. This facile process i) represents a sustainable approach with reusable enzymes, ii) circumvents the drawbacks of in vivo processes while harnessing the selectivity of enzymatic catalysis and iii) helps access an urgently needed drug candidate. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-05 |
AnnouncementXML | Submission_2025-05-05_03:09:07.581.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Thomas Krüger |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Psilocybe sp. 180; NCBI TaxID: 1617910; |
ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2025-02-28 06:10:26 | ID requested | |
⏵ 1 | 2025-05-05 03:09:08 | announced | |
Publication List
Sch, ä, fer T, Sherwood A, Kirkland T, Kr, ü, ger T, Worbs J, Kniemeyer O, Gressler M, Hoffmeister D, In Vitro Psilocybin Synthesis by Co-Immobilized Enzymes. Chemistry, 31(29):e202501037(2025) [pubmed] |
10.1002/chem.202501037; |
Keyword List
submitter keyword: biosynthesis, mushrooms, LC-MS,Psilocybin, in vitro synthesis |
Contact List
Axel Brakhage |
contact affiliation | Department for Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Leibniz-HKI, Jena |
contact email | axel.brakhage@leibniz-hki.de |
lab head | |
Thomas Krüger |
contact affiliation | Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute |
contact email | thomas.krueger@leibniz-hki.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD061303
- Label: PRIDE project
- Name: In Vitro Psilocybin Synthesis by Co-Immobilized Enzymes