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PXD061303

PXD061303 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn Vitro Psilocybin Synthesis by Co-Immobilized Enzymes
DescriptionAdvanced clinical trials investigate the Psilocybe magic mushroom natural product psilocybin as a treatment against major depressive disorder. Currently, synthetic material is used to meet the demand for legitimate pharmaceutical purposes. Here, we report an in vitro approach to biocatalytically produce psilocybin on a solid-phase matrix charged with five covalently bound biosynthetic enzymes. These enzymes include three Psilocybe enzymes: IasA, an engineered L-tryptophan decarboxylase/aromatic aldehyde synthase, the 4-hydroxytryptamine kinase PsiK and the norbaeocystin methyltransferase PsiM, along with Escherichia coli nucleosidase MtnN and adenine deaminase Ade. In a proof-of-principle experiment, this enzyme-charged resin allowed for quantitative turnover of 4-hydroxy-L-tryptophan into psilocybin. This facile process i) represents a sustainable approach with reusable enzymes, ii) circumvents the drawbacks of in vivo processes while harnessing the selectivity of enzymatic catalysis and iii) helps access an urgently needed drug candidate.
HostingRepositoryPRIDE
AnnounceDate2025-05-05
AnnouncementXMLSubmission_2025-05-05_03:09:07.581.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterThomas Krüger
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Psilocybe sp. 180; NCBI TaxID: 1617910;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-28 06:10:26ID requested
12025-05-05 03:09:08announced
Publication List
Sch, ä, fer T, Sherwood A, Kirkland T, Kr, ü, ger T, Worbs J, Kniemeyer O, Gressler M, Hoffmeister D, In Vitro Psilocybin Synthesis by Co-Immobilized Enzymes. Chemistry, 31(29):e202501037(2025) [pubmed]
10.1002/chem.202501037;
Keyword List
submitter keyword: biosynthesis, mushrooms, LC-MS,Psilocybin, in vitro synthesis
Contact List
Axel Brakhage
contact affiliationDepartment for Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Leibniz-HKI, Jena
contact emailaxel.brakhage@leibniz-hki.de
lab head
Thomas Krüger
contact affiliationLeibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
contact emailthomas.krueger@leibniz-hki.de
dataset submitter
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