PXD061302

PXD061302 is an original dataset announced via ProteomeXchange.

Title	Golgin knockouts alter extracellular matrix secretion
Description	The secretion of extracellular matrix (ECM) proteins is vital to the maintenance of tissue health. One 28 major control point of this process is the Golgi apparatus, whose dysfunction causes numerous 29 connective tissue disorders. Golgi function is tightly linked to its structure, which is maintained by the 30 cytoskeleton and Golgi organising proteins. We sought to investigate the role of two of these 31 organising proteins, the golgins GMAP210 and Golgin-160, in ECM secretion. We found that loss of 32 either protein had distinct impacts on Golgi organisation. GMAP210 loss caused cisternal 33 fragmentation and dilation, alongside the accumulation of tubulovesicular structures. Meanwhile, 34 Golgin-160 knockout lead to Golgi fragmentation and vesicle build-up. Nonetheless, loss of each 35 protein had a similar impact on ECM secretion and glycosaminoglycan synthesis. We therefore 36 propose that golgins are collectively required to create the correct physical-chemical space to 37 support efficient ECM protein secretion and modification. This is the first time that Golgin-160 has 38 been shown to be required for ECM secretion.
HostingRepository	PRIDE
AnnounceDate	2025-08-25
AnnouncementXML	Submission_2025-08-24_17:05:27.631.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Hoyle
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-28 04:38:38	ID requested
⏵ 1	2025-08-24 17:05:28	announced

10.1083/jcb.202411167;

Thompson G, Hoyle A, Lewis PA, Prada-Sanchez ME, Swift J, Heesom K, Lowe M, Stephens D, Stevenson NL, Multiple golgins are required to support extracellular matrix secretion, modification, and assembly. J Cell Biol, 224(10):(2025) [pubmed]

submitter keyword: secretion, Extracellular matrix,Golgin

Nicola Stevenson
contact affiliation	University of Bristol
contact email	Nicola.stevenson@bristol.ac.uk
lab head
Anna Hoyle
contact affiliation	University of Oxford
contact email	anna.hoyle@kennedy.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061302
     1. Label: PRIDE project
     2. Name: Golgin knockouts alter extracellular matrix secretion