PXD061298

PXD061298 is an original dataset announced via ProteomeXchange.

Title	Co-translational determination of quaternary structures in chaperone factories
Description	The HSP90/R2TP quaternary chaperone assembles key cellular machines, including the three nuclear RNA polymerases and many non-coding RNPs. Here, we show that the R2TP binds many partners co-translationally. Remarkably, its co-translational interactome reveals many novel potential clients, and side-by-side comparison of RIP-seq and quantitative proteomics identifies clients bound only co-translationally, only post-translationally or both. For pairs of subunits assembling together and bound co-translationally by R2TP, only a marginal proportion of their mRNAs is co-localized and co-translated. Instead, the HSP90 and R2TP chaperones induce the formation of dedicated factories, which are cytoplasmic R2TP foci accumulating client mRNAs, thus favoring co-translational interactions. The R2TP alternates between co- and post-translational steps in a cycle regulated by ATP: following co-translational loading, it accompanies completed subunits towards their partners and becomes released following ATP hydrolysis. Assembly of protein complexes is thus initiated early by chaperones and this mechanism, dubbed co-translational chaperone channeling (cha-cha), substitutes for the rarity of co-localized/co-translated mRNAs.
HostingRepository	PRIDE
AnnounceDate	2026-01-06
AnnouncementXML	Submission_2026-01-06_08:58:07.438.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD061298
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Functional Proteomics Platform FPP
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-28 00:23:03	ID requested
⏵ 1	2026-01-06 08:58:08	announced

10.6019/PXD061298;

submitter keyword: translation factories, chaperone,co-translational assembly

Edouard Bertrand
contact affiliation	UMR9002 CNRS-UM Institute of Human Genetics
contact email	edouard.bertrand@cnrs.fr
lab head
Functional Proteomics Platform FPP
contact affiliation	FPP CNRS
contact email	contact@fpp.cnrs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061298
     1. Label: PRIDE project
     2. Name: Co-translational determination of quaternary structures in chaperone factories