PXD061294

PXD061294 is an original dataset announced via ProteomeXchange.

Title	Protein expression in pancreatic cancer cell lines and normal pancreatic ductal cell line.
Description	Pancreatic cancer (PC) is the sixth leading cause of cancer-associated death worldwide. Despite improvements in treatment methods, the 5-year survival rate of PC patients is still low. Although carbohydrate antigen (CA) 19-9 has been used as the most specific and sensitive biomarker for the diagnosis of PC, approximately 34% of PC patients may have a Lewis antigen-negative phenotype and secrete little, if any, CA19-9. Therefore, it is important to develop effective diagnostic methods for PC. In this study, we performed a global shotgun proteomics analysis using PC cell lines and a normal pancreatic ductal cell line. We identified 142 candidate proteins with differential expression in PC cells. A label-free semiquantitative method based on spectral counting and Gene Ontology analysis led to a total of 25 candidate proteins that could potentially be detected in blood. Validation studies revealed that the expression of fibulin-1 was lower in PC cells than in normal pancreatic ductal cells. Moreover, in vivo fibulin-1 expression was significantly lower in serum from PC patients than in healthy individuals. These findings suggest that lower blood levels of fibulin-1 may be a novel biomarker for the detection of PC.
HostingRepository	jPOST
AnnounceDate	2025-12-03
AnnouncementXML	Submission_2025-12-02_15:48:07.747.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	TETSUSHI YAMAMOTO
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-27 19:10:52	ID requested
⏵ 1	2025-12-02 15:48:08	announced

Yamamoto T, Boku S, Mitamura K, Taga A, Shotgun Label-Free Proteomic Analysis for Identification of a Potential Diagnostic Biomarker for Pancreatic Cancer. Biomedicines, 13(11):(2025) [pubmed]

submitter keyword: pancreatic cancer, shotgun proteomics, protein, diagnosis marker

Tetsushi Yamamoto
lab head
TETSUSHI YAMAMOTO
contact affiliation	Kindai University
dataset submitter

jPOST dataset URI

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