PXD061292
PXD061292 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Pseudomonas aeruginosa manipulates a CNMT-TFR1 methylation pathway to drive macrophage ferroptosis via quorum sensing molecule PQS |
Description | Pseudomonas aeruginosa employs quorum sensing (QS) small molecules such as Pseudomonas Quinolone Signal (PQS) to manipulate host cell death. Although PQS triggers several cell death modalities, its role in ferroptosis remains enigmatic. Here, we reported that PQS induces ferroptosis in macrophages through a previously unrecognized carnosine-N-methyltransferase (CNMT)-transferrin receptor 1 (TFR1) methylation pathway. Using thermal proteome profilingscreening, we identified CNMT as the direct intracellular receptor of PQS in macrophage. Mechanistically, PQS binding activates CNMT’s histidine methyltransferase activity, catalyzing H35 methylation on TFR1. This methylation modification increases TFR1 expressions, amplifying iron acquisition and lipid peroxidation for ferroptosis. Crucially, the PQS-CNMT-TFR1 axis is distinct from canonical bacterial pathogens that exploit host cell death pathways, revealing P. aeruginosa’s unique strategy to exploit host epigenetic machinery. Our findings redefine QS molecules as mediators of interkingdom signaling and position CNMT-TFR1 methylation as therapeutic targets aimed at blocking ferroptosis-driven immunopathology to mitigate P. aeruginosa virulence. |
HostingRepository | iProX |
AnnounceDate | 2025-02-27 |
AnnouncementXML | Submission_2025-02-27_18:06:27.906.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fengming Li |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2025-02-27 18:06:15 | ID requested | |
⏵ 1 | 2025-02-27 18:06:28 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: PQS, RAW264.7, CNMT, ferroptosis |
Contact List
Chris Soon Heng Tan | |
---|---|
contact affiliation | Southern University of Science and Technology |
contact email | christan@sustech.edu.cn |
lab head | |
Fengming Li | |
contact affiliation | Southern University of Science and Technology |
contact email | 12331077@mail.sustech.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |