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PXD061292

PXD061292 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePseudomonas aeruginosa manipulates a CNMT-TFR1 methylation pathway to drive macrophage ferroptosis via quorum sensing molecule PQS
DescriptionPseudomonas aeruginosa employs quorum sensing (QS) small molecules such as Pseudomonas Quinolone Signal (PQS) to manipulate host cell death. Although PQS triggers several cell death modalities, its role in ferroptosis remains enigmatic. Here, we reported that PQS induces ferroptosis in macrophages through a previously unrecognized carnosine-N-methyltransferase (CNMT)-transferrin receptor 1 (TFR1) methylation pathway. Using thermal proteome profilingscreening, we identified CNMT as the direct intracellular receptor of PQS in macrophage. Mechanistically, PQS binding activates CNMT’s histidine methyltransferase activity, catalyzing H35 methylation on TFR1. This methylation modification increases TFR1 expressions, amplifying iron acquisition and lipid peroxidation for ferroptosis. Crucially, the PQS-CNMT-TFR1 axis is distinct from canonical bacterial pathogens that exploit host cell death pathways, revealing P. aeruginosa’s unique strategy to exploit host epigenetic machinery. Our findings redefine QS molecules as mediators of interkingdom signaling and position CNMT-TFR1 methylation as therapeutic targets aimed at blocking ferroptosis-driven immunopathology to mitigate P. aeruginosa virulence.
HostingRepositoryiProX
AnnounceDate2025-02-27
AnnouncementXMLSubmission_2025-02-27_18:06:27.906.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFengming Li
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-27 18:06:15ID requested
12025-02-27 18:06:28announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PQS, RAW264.7, CNMT, ferroptosis
Contact List
Chris Soon Heng Tan
contact affiliationSouthern University of Science and Technology
contact emailchristan@sustech.edu.cn
lab head
Fengming Li
contact affiliationSouthern University of Science and Technology
contact email12331077@mail.sustech.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI