PXD061281

PXD061281 is an original dataset announced via ProteomeXchange.

Title	Analysis of Rev1-dependent replication dynamics using isolation of proteins on nascent DNA
Description	To study the influence of the Rev1 translesion synthesis enzyme on fork dynamics, we performed isolation of proteins on nascent DNA coupled with mass spectrometry (iPOND-MS). Wild-type and REV1-KO HEK293 Flp-In™/T-REx™ cells were used. Treatment with the G-quadruplex stabilizing agent pyridostatin (PDS; 10 micromolar for either 15 or 60 minutes) was used to examine the Rev1-dependent response to G4-induced replication stress. A thymidine pulse experiment was used to ensure that replication proteins were enriched in the samples pulsed with 5-ethynyl-2ʹ-deoxyuridine (EdU). The resulting samples were analyzed using liquid chromatography mass spectrometry (LC-MS). Label-free quantitative analysis of peptide abundance was used to determine changes in response to Rev1 deletion and treatment with PDS.
HostingRepository	PRIDE
AnnounceDate	2026-06-15
AnnouncementXML	Submission_2026-06-14_17:45:06.852.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD061281
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dennis Province
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-27 12:28:42	ID requested
⏵ 1	2026-06-14 17:45:07	announced

Ketkar A, Paxton BC, Zuniga OE, Sewilam RS, Morales M, Schaller J, McCollum RJ, Jackson KE, Lowran K, Paul A, Patel M, Seenivasan S, McCrury M, Al-Anbaky Q, Maddukuri L, Kendrick S, Wu CG, Gunderson JEC, Eoff RL, Human REV1 interacts with DHX36 to promote replication and tolerance of G-quadruplex DNA. Nucleic Acids Res, 54(11):(2026) [pubmed]

10.1093/nar/gkag562;

10.6019/PXD061281;

submitter keyword: DNA replication, G-quadruplex, translesion synthesis

Robert L. Eoff
contact affiliation	University of Arkansas for Medical Sciences
contact email	RLEOFF@UAMS.EDU
lab head
Dennis Province
contact affiliation	UAMS
contact email	dprovince@uams.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD061281

PRIDE project URI

• PRIDE
  1. PXD061281
     1. Label: PRIDE project
     2. Name: Analysis of Rev1-dependent replication dynamics using isolation of proteins on nascent DNA