PXD061239

PXD061239 is an original dataset announced via ProteomeXchange.

Title	An H3K4me3 reader recruits the positive transcription elongation factor B complex to facilitate transcription elongation
Description	Transcription by RNA polymerase II (Pol II) is a tightly regulated process in eukaryotes. A recent study revealed that the trimethylation of histone H3 at lysine 4 (H3K4me3) activates gene expression by promoting Pol II pause-release and transcription elongation rather than transcription initiation. However, the molecular mechanisms linking H3K4me3 to transcription elongation remain largely unclear. Here, we show that an H3K4me3 reader recruits the positive transcription elongation factor B (P-TEFb) complex to regulate this process. Genetic screenings in Arabidopsis reveal that RGSA1 is required for the expression of genes induced by the plant hormone salicylic acid. RGSA1 encodes an H3K4me3 reader containing a cysteine-tryptophan domain critical for its function. RGSA1 mainly occupies transcription start sites, where it recruits P-TEFb to promote the release of paused Pol II and facilitate transcription elongation. Given the evolutionary conservation of H3K4me3 readers and P-TEFb, our findings may represent a conserved mechanism underlying transcription elongation across eukaryotes.
HostingRepository	PRIDE
AnnounceDate	2026-01-27
AnnouncementXML	Submission_2026-01-26_19:47:43.382.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaodan Yu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-26 18:45:59	ID requested
⏵ 1	2026-01-26 19:47:43	announced

Dataset with its publication pending

submitter keyword: Transcription elongation

H3K4me3

P-TEFb

salicylic acid

Arabidopsis

root

Xiaodan Yu
contact affiliation	College of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
contact email	ppaofish@126.com
lab head
Xiaodan Yu
contact affiliation	none
contact email	ppaofish@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD061239
     1. Label: PRIDE project
     2. Name: An H3K4me3 reader recruits the positive transcription elongation factor B complex to facilitate transcription elongation