PXD061238

PXD061238 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry analysis of colonic neutrophils in colitis
Description	Neutrophils are essential for microbial defense but can aggravate tissue damage by prolonged recruitment and activation. The complexity of neutrophil involvement in chronic inflammation underscores our limited understanding of how their adaptation and reprogramming in tissues influence disease progression. Here, we demonstrate that neutrophils recruited in acute inflammatory bowel disease (IBD), chronic granulomatous disease-associated IBD, and in murine colitis and infection models undergo rapid and extensive transcriptional reprogramming in the intestine, resulting in the emergence of DUOX2 NADPH oxidase expression. Functional studies utilizing neutrophil-specific DUOX2-inactivated mice reveal critical and dichotomous roles of DUOX2 in promoting inflammation during colitis while protecting against intestinal infection. Neutrophils with active DUOX2 amplify and prolong inflammatory responses, contributing to a more proinflammatory milieu in the intestine. These findings unveil a niche-directed reprogramming of neutrophils that orchestrates the immune response during inflammation, presenting a novel and promising target for therapeutic development in IBD.
HostingRepository	PRIDE
AnnounceDate	2025-05-20
AnnouncementXML	Submission_2025-05-20_07:13:34.762.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ashish Singh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-26 18:33:51	ID requested
⏵ 1	2025-05-20 07:13:35	announced

10.1073/PNAS.2421747122;

submitter keyword: LC-MSMS, Colitis,Neutrophil

Ulla Knaus
contact affiliation	Conway Institute, School of Medicine, University College Dublin, Ireland
contact email	ulla.knaus@ucd.ie
lab head
Ashish Singh
contact affiliation	University College Dublin
contact email	singhashish1186@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061238

PRIDE project URI

• PRIDE
  1. PXD061238
     1. Label: PRIDE project
     2. Name: Mass spectrometry analysis of colonic neutrophils in colitis