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PXD061237

PXD061237 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic insights into novel food insects: Homology-based proteome characterization and allergenicity considerations for EU-regulated insect species
DescriptionInsects have emerged as a sustainable alternative protein source and recently gained regulatory approval in the European Union as novel foods and animal feed ingredients. However, the limited availability of species-specific proteomic databases presents a significant challenge for the accurate identification of proteins in insect-based food products, relevant for food authenticity, safety and allergenicity concerns. In this study, we developed a homology-based proteomic workflow to address the lack of comprehensive sequence data for edible insect species and to explore their allergenic potential. This nanoflow tandem mass spectrometry method (nano-HPLC HR-MS) aims to be generally applicable to underexplored species. We identified between 1079 and 1893 proteins for each of the six investigated insect species Acheta domesticus, Locusta migratoria, Tenebrio molitor, Alphitobius diaperinus, Gryllodes sigillatus and Hermetia illucens, increasing their numbers of known proteins up to 24-fold. Furthermore, we assessed the influence of error-tolerant peptide-spectra matching searches and database composition on protein identification and confirmed on average 90% of selected peptides using targeted methods. We consistently detected known and putative allergens across all tested species, providing further evidence on their allergenic potential. Data from this study may provide the general basis for the development of targeted parallel reaction monitoring mass spectrometry-based assays for so far unsequenced species authentication and quantification of their proteins e.g. in novel foods.
HostingRepositoryPRIDE
AnnounceDate2025-06-03
AnnouncementXMLSubmission_2025-06-03_08:27:07.701.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD061237
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTobias Meisinger
SpeciesList scientific name: Tenebrio molitor; NCBI TaxID: 7067; scientific name: Alphitobius diaperinus; NCBI TaxID: 27448; scientific name: Hermetia illucens; NCBI TaxID: 343691; scientific name: Gryllodes sigillatus; NCBI TaxID: 13551; scientific name: Acheta domesticus; NCBI TaxID: 6997; scientific name: Locusta migratoria; NCBI TaxID: 7004;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02025-02-26 17:43:08ID requested
12025-06-03 08:27:08announced
Publication List
10.6019/PXD061237;
10.1016/J.FOODCONT.2025.111441;
Keyword List
submitter keyword: allergen,insect, LC-MS/MS
Contact List
Oliver Poetz
contact affiliationSIGNATOPE GmbH, Markwiesenstraße 55, 72770 Reutlingen, Germany
contact emailpoetz@signatope.com
lab head
Tobias Meisinger
contact affiliationSIGNATOPE GmbH
contact emailmeisinger@signatope.com
dataset submitter
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Dataset FTP location
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