PXD061235

PXD061235 is an original dataset announced via ProteomeXchange.

Title	PUS10-induced tRNA fragmentation impacts retrotransposon-driven inflammation
Description	Pseudouridine synthases (PUSs) catalyze the isomerization of uridine (U)-to-pseudouridine (Ψ) and have emerging roles in development and disease. How PUSs adapt gene expression under stress remains mostly unexplored. We identify an unconventional role for the Ψ “writer” PUS10 impacting intracellular innate immunity. Using Pus10 knock-out mice, we uncover cell-intrinsic upregulation of interferon (IFN) signalling, conferring resistance to inflammation in vivo. Pus10 loss alters tRNA-derived small RNAs (tdRs) abundance, perturbing translation and endogenous retroelements expression. We found one of the differentially expressed tDRs is derived from tRNA glycine (tDR Gly). To delinate the tDR Gly interactome, we employed an optimized pull-down strategy using PUS10 KO cells followed by data-independent acquisition-based quantitative mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2025-05-27
AnnouncementXML	Submission_2025-05-27_06:04:36.143.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sudip Ghosh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-26 14:43:25	ID requested
⏵ 1	2025-05-27 06:04:36	announced

10.1016/J.CELREP.2025.115735;

submitter keyword: PUS10, quantitative mass spectrometry, data-independent acquisition, tRNA-derived small RNAs

Jenny Hansson
contact affiliation	Proteomic Hematology, Department of Experimental Medical Science, Lund Stem Cell Center, Lund University
contact email	jenny.hansson@med.lu.se
lab head
Sudip Ghosh
contact affiliation	Proteomic Hematology, Department of Experimental Medical Science, Lund Stem Cell Center, Lund University
contact email	sudip.ghosh@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD061235

PRIDE project URI

• PRIDE
  1. PXD061235
     1. Label: PRIDE project
     2. Name: PUS10-induced tRNA fragmentation impacts retrotransposon-driven inflammation