PXD061217

PXD061217 is an original dataset announced via ProteomeXchange.

Title	Salmonella effector kinase SteC is activated by host-mediated phosphorylation
Description	The pathogen Salmonella, which causes significant human morbidity and mortality, encodes an effector kinase, SteC, which mediates actin polymerisation and cell migration of the infected cell. Analysis of the sequence and predicted structure alongside canonical eukaryotic serine-threonine kinases raises the questions of how it is catalytically active and how this activity is regulated. Here, we reveal that SteC is activated following the phosphorylation of a highly conserved residue, S379, by a host kinase. This induces a dramatic increase in SteC nucleotide binding affinity, providing an explanation for S379’s requirement for substrate phosphorylation and actin polymerisation. Further mutational analysis revealed HD and DGD motifs within the depleted C lobe that are important for function, and may represent non-canonical mimics of HxD and DFG motifs in eukaryotic serine/threonine kinases. Meanwhile, the C-tail of SteC, encompassing amino acids 429-457, is essential for function following translocation from Salmonella, even though it is not required for catalysis in vitro. Overall, our findings uncover two previously unappreciated mechanisms that mediate the activity of the only Salmonella effector kinase within the host.
HostingRepository	PRIDE
AnnounceDate	2026-06-12
AnnouncementXML	Submission_2026-06-12_05:09:45.019.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sarah Maslen
SpeciesList	scientific name: Salmonella enterica subsp. enterica serovar Typhimurium str. T000240; NCBI TaxID: NEWT:718274;
ModificationList	No PTMs are included in the dataset
Instrument	SELECT SERIES Cyclic IMS

Revision	Datetime	Status	ChangeLog Entry
0	2025-02-26 02:00:10	ID requested
⏵ 1	2026-06-12 05:09:45	announced

Dataset with its publication pending

submitter keyword: bacterial kinase, HDX, SteC, effector, phosphorylation, Salmonella,Host-pathogen interactions, kinase regulation, non-RD kinase

Dr. Katrin Rittinger
contact affiliation	Molecular Structure of Cell Signalling Laboratory, The Francis Crick Institute, 1 Midland Road, London, NW1 1AT, UK
contact email	katrin.rittinger@crick.ac.uk
lab head
Sarah Maslen
contact affiliation	The Francis Crick Institute
contact email	sarah.maslen@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD061217

PRIDE project URI

• PRIDE
  1. PXD061217
     1. Label: PRIDE project
     2. Name: Salmonella effector kinase SteC is activated by host-mediated phosphorylation